Protein labeling approach to improve lysosomal targeting and efficacy of antibody-drug conjugates

Ying Han; Yifan Da; Mingjia Yu; Yaping Cheng; Xin Wang; Jiale Xiong; Guoying Guo; Yan Li; Xianxing Jiang; Xiaoqing Cai

doi:10.1039/d0ob00265h

Protein labeling approach to improve lysosomal targeting and efficacy of antibody-drug conjugates

Ying Han
, Yifan Da
, Mingjia Yu
, Yaping Cheng
, Xin Wang
, Jiale Xiong
, Guoying Guo
, Yan Li
, Xianxing Jiang
, Xiaoqing Cai^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

An anti-EGFR nanobody was labeled at the C-terminus with a lysosome-sorting NPGY (Asn-Pro-Gly-Tyr) motifviasortase-mediated ligation to enhance the engagement of the clathrin-mediated endocytosis. The synergistic effects of NPGY motif and nona-arginine peptide were found to induce robust internalization and lysosomal trafficking, which in turn improved anti-tumor activity of an antibody-drug conjugate.

Original language	English
Pages (from-to)	3229-3233
Number of pages	5
Journal	Organic and Biomolecular Chemistry
Volume	18
Issue number	17
DOIs	https://doi.org/10.1039/d0ob00265h
State	Published - 7 May 2020
Externally published	Yes

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title = "Protein labeling approach to improve lysosomal targeting and efficacy of antibody-drug conjugates",

abstract = "An anti-EGFR nanobody was labeled at the C-terminus with a lysosome-sorting NPGY (Asn-Pro-Gly-Tyr) motifviasortase-mediated ligation to enhance the engagement of the clathrin-mediated endocytosis. The synergistic effects of NPGY motif and nona-arginine peptide were found to induce robust internalization and lysosomal trafficking, which in turn improved anti-tumor activity of an antibody-drug conjugate.",

author = "Ying Han and Yifan Da and Mingjia Yu and Yaping Cheng and Xin Wang and Jiale Xiong and Guoying Guo and Yan Li and Xianxing Jiang and Xiaoqing Cai",

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year = "2020",

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doi = "10.1039/d0ob00265h",

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T1 - Protein labeling approach to improve lysosomal targeting and efficacy of antibody-drug conjugates

AU - Han, Ying

AU - Da, Yifan

AU - Yu, Mingjia

AU - Cheng, Yaping

AU - Wang, Xin

AU - Xiong, Jiale

AU - Guo, Guoying

AU - Li, Yan

AU - Jiang, Xianxing

AU - Cai, Xiaoqing

PY - 2020/5/7

Y1 - 2020/5/7

N2 - An anti-EGFR nanobody was labeled at the C-terminus with a lysosome-sorting NPGY (Asn-Pro-Gly-Tyr) motifviasortase-mediated ligation to enhance the engagement of the clathrin-mediated endocytosis. The synergistic effects of NPGY motif and nona-arginine peptide were found to induce robust internalization and lysosomal trafficking, which in turn improved anti-tumor activity of an antibody-drug conjugate.

AB - An anti-EGFR nanobody was labeled at the C-terminus with a lysosome-sorting NPGY (Asn-Pro-Gly-Tyr) motifviasortase-mediated ligation to enhance the engagement of the clathrin-mediated endocytosis. The synergistic effects of NPGY motif and nona-arginine peptide were found to induce robust internalization and lysosomal trafficking, which in turn improved anti-tumor activity of an antibody-drug conjugate.

UR - https://www.scopus.com/pages/publications/85084272946

U2 - 10.1039/d0ob00265h

DO - 10.1039/d0ob00265h

M3 - 文章

C2 - 32108212

AN - SCOPUS:85084272946

SN - 1477-0520

VL - 18

SP - 3229

EP - 3233

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 17

ER -

Protein labeling approach to improve lysosomal targeting and efficacy of antibody-drug conjugates

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