Promotion of granular activated carbon on methanogenesis of readily acidogenic carbohydrate-rich waste at low inoculation ratio

Although granular activated carbon (GAC) supplementation into food waste anaerobic digestion system is an efficient means to enhance methane production. As yet, little is known whether GAC supplementation is suitable for the extreme condition of pH below 4.5, which occurs in the use of readily acidogenic carbohydrate-rich waste (RACW) as methanogenic substrate when at low inoculation/substrate (I/S) ratio. This study investigated the effects of GAC on RACW anaerobic digestion under different inoculation/substrate (I/S) ratios. It was found that the addition of GAC was a preferred alternative method to enhancing I/S ratio for promoting methane production from RACW. The additive dose of 20 g/L was recommended for the methanogenesis of RACW at low I/S of 1:2, and the methane yield was enhanced by 12 times (505 mL/g-VS) compared with that (42 mL/g-VS) from the control. This promotion resulted from the apparently solving the over-acidogenesis problem and the adjustment of pH to the desired range. Further investigation revealed that the added GAC enhanced the activities of acetate kinase and coenzyme F₄₂₀, that engaged in the acidogenic and methanogenic reactions. Meanwhile, the decrease of hydrogenase and increase of c-Cyts implied that the metabolism of direct interspecies electron transfer (DIET) was probably stimulated by GAC. Microbial investigation inferred that the enriched hydrogenotrophic methanogens and DIET-mediated syntrophic partners of Geobacter/Syntrophomonas with Methanosarcina were responsible for the enhanced methane yield.