Progress on the reaction-based methods for detection of endogenous hydrogen sulfide

Hydrogen sulfide (H₂S) is a biologically signaling molecule that mediates a wide range of physiological functions, which is frequently misregulated in numerous pathological processes. As such, measurement of H₂S holds great attention due to its unique physiological and pathophysiological roles. Currently, a variety of methods based on the H₂S-involved reactions have been reported for detection of endogenous H₂S, bearing the advantages of good specificity and high sensitivity. This review describes in detail the types of reactions, their mechanisms, and their applications in biological research, thus hopefully providing some guidelines to the researchers in this field for further investigation.