Preservation of DNA Nanostructure Carriers: Effects of Freeze-Thawing and Ionic Strength during Lyophilization and Storage

Bing Zhu; Yan Zhao; Jiangbing Dai; Jianbang Wang; Shu Xing; Linjie Guo; Nan Chen; Xiangmeng Qu; Li Li; Juwen Shen; Jiye Shi; Jiang Li; Lihua Wang

doi:10.1021/acsami.7b04784

Preservation of DNA Nanostructure Carriers: Effects of Freeze-Thawing and Ionic Strength during Lyophilization and Storage

Bing Zhu
, Yan Zhao
, Jiangbing Dai
, Jianbang Wang
, Shu Xing
, Linjie Guo
, Nan Chen
, Xiangmeng Qu
, Li Li
, Juwen Shen
, Jiye Shi
, Jiang Li^*
, Lihua Wang

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

DNA nanostructures have attracted wide interest in biomedical applications, especially as nanocarriers for drug delivery. Therefore, it is important to ensure the structural integrity of DNA nanostructures under ambient temperature storage. In this study, we examined lyophilization-based preservation of DNA nanostructures by investigating the structural integrity of different DNA nanostructures reconstituted from lyophilization. We demonstrated that lyophilization under appropriate ionic strength is amenable to the storage of DNA nanostructures. Compared with that stored in liquid solution, DNA nanostructure carriers reconstituted from lyophilization showed significantly better structural integrity after an accelerated aging test equivalent to 100-day room-temperature storage.

Original language	English
Pages (from-to)	18434-18439
Number of pages	6
Journal	ACS Applied Materials and Interfaces
Volume	9
Issue number	22
DOIs	https://doi.org/10.1021/acsami.7b04784
State	Published - 7 Jun 2017

Keywords

DNA nanostructures
ionic strength
lyophilization
storage
structure friendly

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10.1021/acsami.7b04784

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title = "Preservation of DNA Nanostructure Carriers: Effects of Freeze-Thawing and Ionic Strength during Lyophilization and Storage",

abstract = "DNA nanostructures have attracted wide interest in biomedical applications, especially as nanocarriers for drug delivery. Therefore, it is important to ensure the structural integrity of DNA nanostructures under ambient temperature storage. In this study, we examined lyophilization-based preservation of DNA nanostructures by investigating the structural integrity of different DNA nanostructures reconstituted from lyophilization. We demonstrated that lyophilization under appropriate ionic strength is amenable to the storage of DNA nanostructures. Compared with that stored in liquid solution, DNA nanostructure carriers reconstituted from lyophilization showed significantly better structural integrity after an accelerated aging test equivalent to 100-day room-temperature storage.",

keywords = "DNA nanostructures, ionic strength, lyophilization, storage, structure friendly",

author = "Bing Zhu and Yan Zhao and Jiangbing Dai and Jianbang Wang and Shu Xing and Linjie Guo and Nan Chen and Xiangmeng Qu and Li Li and Juwen Shen and Jiye Shi and Jiang Li and Lihua Wang",

note = "Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

year = "2017",

month = jun,

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doi = "10.1021/acsami.7b04784",

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TY - JOUR

T1 - Preservation of DNA Nanostructure Carriers

T2 - Effects of Freeze-Thawing and Ionic Strength during Lyophilization and Storage

AU - Zhu, Bing

AU - Zhao, Yan

AU - Dai, Jiangbing

AU - Wang, Jianbang

AU - Xing, Shu

AU - Guo, Linjie

AU - Chen, Nan

AU - Qu, Xiangmeng

AU - Li, Li

AU - Shen, Juwen

AU - Shi, Jiye

AU - Li, Jiang

AU - Wang, Lihua

PY - 2017/6/7

Y1 - 2017/6/7

N2 - DNA nanostructures have attracted wide interest in biomedical applications, especially as nanocarriers for drug delivery. Therefore, it is important to ensure the structural integrity of DNA nanostructures under ambient temperature storage. In this study, we examined lyophilization-based preservation of DNA nanostructures by investigating the structural integrity of different DNA nanostructures reconstituted from lyophilization. We demonstrated that lyophilization under appropriate ionic strength is amenable to the storage of DNA nanostructures. Compared with that stored in liquid solution, DNA nanostructure carriers reconstituted from lyophilization showed significantly better structural integrity after an accelerated aging test equivalent to 100-day room-temperature storage.

AB - DNA nanostructures have attracted wide interest in biomedical applications, especially as nanocarriers for drug delivery. Therefore, it is important to ensure the structural integrity of DNA nanostructures under ambient temperature storage. In this study, we examined lyophilization-based preservation of DNA nanostructures by investigating the structural integrity of different DNA nanostructures reconstituted from lyophilization. We demonstrated that lyophilization under appropriate ionic strength is amenable to the storage of DNA nanostructures. Compared with that stored in liquid solution, DNA nanostructure carriers reconstituted from lyophilization showed significantly better structural integrity after an accelerated aging test equivalent to 100-day room-temperature storage.

KW - DNA nanostructures

KW - ionic strength

KW - lyophilization

KW - storage

KW - structure friendly

UR - https://www.scopus.com/pages/publications/85020239410

U2 - 10.1021/acsami.7b04784

DO - 10.1021/acsami.7b04784

M3 - 文章

C2 - 28547989

AN - SCOPUS:85020239410

SN - 1944-8244

VL - 9

SP - 18434

EP - 18439

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 22

ER -

Preservation of DNA Nanostructure Carriers: Effects of Freeze-Thawing and Ionic Strength during Lyophilization and Storage

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