Polymorphic microsatellite loci for the razor clam, Sinonovacula constricta

H. T. Ma; H. B. Jiang; X. Q. Liu; X. P. Wu; X. M. Wei

doi:10.4238/2015.January.15.17

Polymorphic microsatellite loci for the razor clam, Sinonovacula constricta

H. T. Ma
, H. B. Jiang
, X. Q. Liu^*
, X. P. Wu
, X. M. Wei

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)₁₆, (GA)₁₆. Polymorphisms of these loci were evaluated in a wild population of 30 individuals. The allele number of these polymorphic markers ranged from 5-15 per locus with an average of 9.333. Observed and expected heterozygosity values ranged from 0.192-1.000 and 0.219-0.906. Polymorphism information content ranged from 0.209-0.892 with an average of 0.704. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected between these loci. This set of microsatellite loci are useful for genetic studies in S. constricta.

Original language	English
Pages (from-to)	145-148
Number of pages	4
Journal	Genetics and Molecular Research
Volume	14
Issue number	1
DOIs	https://doi.org/10.4238/2015.January.15.17
State	Published - 15 Jan 2015
Externally published	Yes

Keywords

Microsatellites
Polymorphism
Sinonovacula constricta

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10.4238/2015.January.15.17

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title = "Polymorphic microsatellite loci for the razor clam, Sinonovacula constricta",

abstract = "The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)16, (GA)16. Polymorphisms of these loci were evaluated in a wild population of 30 individuals. The allele number of these polymorphic markers ranged from 5-15 per locus with an average of 9.333. Observed and expected heterozygosity values ranged from 0.192-1.000 and 0.219-0.906. Polymorphism information content ranged from 0.209-0.892 with an average of 0.704. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected between these loci. This set of microsatellite loci are useful for genetic studies in S. constricta.",

keywords = "Microsatellites, Polymorphism, Sinonovacula constricta",

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note = "Publisher Copyright: {\textcopyright} FUNPEC-RP.",

year = "2015",

month = jan,

day = "15",

doi = "10.4238/2015.January.15.17",

language = "英语",

volume = "14",

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TY - JOUR

T1 - Polymorphic microsatellite loci for the razor clam, Sinonovacula constricta

AU - Ma, H. T.

AU - Jiang, H. B.

AU - Liu, X. Q.

AU - Wu, X. P.

AU - Wei, X. M.

N1 - Publisher Copyright: © FUNPEC-RP.

PY - 2015/1/15

Y1 - 2015/1/15

N2 - The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)16, (GA)16. Polymorphisms of these loci were evaluated in a wild population of 30 individuals. The allele number of these polymorphic markers ranged from 5-15 per locus with an average of 9.333. Observed and expected heterozygosity values ranged from 0.192-1.000 and 0.219-0.906. Polymorphism information content ranged from 0.209-0.892 with an average of 0.704. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected between these loci. This set of microsatellite loci are useful for genetic studies in S. constricta.

AB - The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)16, (GA)16. Polymorphisms of these loci were evaluated in a wild population of 30 individuals. The allele number of these polymorphic markers ranged from 5-15 per locus with an average of 9.333. Observed and expected heterozygosity values ranged from 0.192-1.000 and 0.219-0.906. Polymorphism information content ranged from 0.209-0.892 with an average of 0.704. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected between these loci. This set of microsatellite loci are useful for genetic studies in S. constricta.

KW - Microsatellites

KW - Polymorphism

KW - Sinonovacula constricta

UR - https://www.scopus.com/pages/publications/84921291845

U2 - 10.4238/2015.January.15.17

DO - 10.4238/2015.January.15.17

M3 - 文章

C2 - 25729945

AN - SCOPUS:84921291845

SN - 1676-5680

VL - 14

SP - 145

EP - 148

JO - Genetics and Molecular Research

JF - Genetics and Molecular Research

IS - 1

ER -

Polymorphic microsatellite loci for the razor clam, Sinonovacula constricta

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Keywords

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