TY - JOUR
T1 - Poly(ethylene glycol)-Mediated Assembly of Vaccine Particles to Improve Stability and Immunogenicity
AU - Zhang, Guiqiang
AU - Fu, Xiao
AU - Sun, Haifeng
AU - Zhang, Peiyu
AU - Zhai, Shumei
AU - Hao, Jingcheng
AU - Cui, Jiwei
AU - Hu, Ming
N1 - Publisher Copyright:
©
PY - 2021/3/31
Y1 - 2021/3/31
N2 - We report the one-step assembly of vaccine particles by encapsulating ovalbumin (OVA) and cytosine-phosphate-guanine oligodeoxynucleotides (CpG) into poly(ethylene glycol) (PEG)-mediated zeolitic imidazolate framework-8 nanoparticles (OVA-CpG@ZIF-8 NPs), where PEG improves the stability and dispersity of ZIF-8 NPs and the NPs protect the encapsulated OVA and CpG to circumvent the cold chain issue. Compared with free OVA and OVA-encapsulated ZIF-8 (OVA@ZIF-8) NPs, OVA-CpG@ZIF-8 NPs can enhance antigen uptake, cross-presentation, dendritic cell (DC) maturation, production of specific antibody and cytokines, and CD4+ T and CD8+ T cell activation. More importantly, the vaccine particles retain their bioactivity against enzymatic degradation, elevated temperatures, and long-term storage at ambient temperature. The study highlights the importance of PEG-mediated ZIF-8 NPs as a vaccine delivery system for the promising application of effective and cold chain-independent vaccination against diseases.
AB - We report the one-step assembly of vaccine particles by encapsulating ovalbumin (OVA) and cytosine-phosphate-guanine oligodeoxynucleotides (CpG) into poly(ethylene glycol) (PEG)-mediated zeolitic imidazolate framework-8 nanoparticles (OVA-CpG@ZIF-8 NPs), where PEG improves the stability and dispersity of ZIF-8 NPs and the NPs protect the encapsulated OVA and CpG to circumvent the cold chain issue. Compared with free OVA and OVA-encapsulated ZIF-8 (OVA@ZIF-8) NPs, OVA-CpG@ZIF-8 NPs can enhance antigen uptake, cross-presentation, dendritic cell (DC) maturation, production of specific antibody and cytokines, and CD4+ T and CD8+ T cell activation. More importantly, the vaccine particles retain their bioactivity against enzymatic degradation, elevated temperatures, and long-term storage at ambient temperature. The study highlights the importance of PEG-mediated ZIF-8 NPs as a vaccine delivery system for the promising application of effective and cold chain-independent vaccination against diseases.
KW - cold chain
KW - immunogenicity
KW - metal-organic frameworks
KW - poly(ethylene glycol)
KW - vaccine delivery
UR - https://www.scopus.com/pages/publications/85103684077
U2 - 10.1021/acsami.1c00706
DO - 10.1021/acsami.1c00706
M3 - 文章
C2 - 33749241
AN - SCOPUS:85103684077
SN - 1944-8244
VL - 13
SP - 13978
EP - 13989
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 12
ER -
