Platinum particles-modified electrode for HPLC with pulsed amperometric detection of thiols in rat striatum

Xu Ni Cao; Jin Hua Li; Hai Hong Xu; Li Lin; Yue Zhong Xian; Katsunobu Yamamoto; Li Tong Jin

doi:10.1002/bmc.357

Platinum particles-modified electrode for HPLC with pulsed amperometric detection of thiols in rat striatum

Xu Ni Cao
, Jin Hua Li
, Hai Hong Xu
, Li Lin
, Yue Zhong Xian
, Katsunobu Yamamoto
, Li Tong Jin^*

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A new chemically modified electrode based on the immobilization of Pt particles is fabricated and exhibits electrocatalytic oxidation for L-cysteine (L-Cys), glutathione (GSH) and penicillamine (PEN) with relatively high sensitivity. It is also adaptable to HPLC for pulsed amperometric detection (PAD) of these thiols. PAD largely improves the detection sensitivity because the alternated polarizations can effectively clean and reactivate the electrode surface. It is shown that the peak currents of L-Cys, GSH and PEN are linear to their concentrations, with the calculated detection limit of 1.1 × 10^-7, 1.8 × 10^-7 and 3.8 × 10^-7 mol L^-1, respectively (S/N = 3). The method has been successfully applied to assess the contents of L-Cys and GSH in rat striatal microdialysates. The average contents of the two analytes in rat striatum are 2.6 × 10^-6 and 2.8 × 10^-6 mol L^-1, respectively.

Original language	English
Pages (from-to)	564-569
Number of pages	6
Journal	Biomedical Chromatography
Volume	18
Issue number	8
DOIs	https://doi.org/10.1002/bmc.357
State	Published - Oct 2004

Keywords

Glutathione
L-cysteine
Penicillamine
Pt particles
Pulsed amperometric detection
Striatum

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@article{48b9368ad9c746718de4201006d92e2e,

title = "Platinum particles-modified electrode for HPLC with pulsed amperometric detection of thiols in rat striatum",

abstract = "A new chemically modified electrode based on the immobilization of Pt particles is fabricated and exhibits electrocatalytic oxidation for L-cysteine (L-Cys), glutathione (GSH) and penicillamine (PEN) with relatively high sensitivity. It is also adaptable to HPLC for pulsed amperometric detection (PAD) of these thiols. PAD largely improves the detection sensitivity because the alternated polarizations can effectively clean and reactivate the electrode surface. It is shown that the peak currents of L-Cys, GSH and PEN are linear to their concentrations, with the calculated detection limit of 1.1 × 10-7, 1.8 × 10-7 and 3.8 × 10-7 mol L-1, respectively (S/N = 3). The method has been successfully applied to assess the contents of L-Cys and GSH in rat striatal microdialysates. The average contents of the two analytes in rat striatum are 2.6 × 10-6 and 2.8 × 10-6 mol L-1, respectively.",

keywords = "Glutathione, L-cysteine, Penicillamine, Pt particles, Pulsed amperometric detection, Striatum",

author = "Cao, \{Xu Ni\} and Li, \{Jin Hua\} and Xu, \{Hai Hong\} and Li Lin and Xian, \{Yue Zhong\} and Katsunobu Yamamoto and Jin, \{Li Tong\}",

year = "2004",

month = oct,

doi = "10.1002/bmc.357",

language = "英语",

volume = "18",

pages = "564--569",

journal = "Biomedical Chromatography",

issn = "0269-3879",

publisher = "John Wiley and Sons Ltd",

number = "8",

}

TY - JOUR

T1 - Platinum particles-modified electrode for HPLC with pulsed amperometric detection of thiols in rat striatum

AU - Cao, Xu Ni

AU - Li, Jin Hua

AU - Xu, Hai Hong

AU - Lin, Li

AU - Xian, Yue Zhong

AU - Yamamoto, Katsunobu

AU - Jin, Li Tong

PY - 2004/10

Y1 - 2004/10

N2 - A new chemically modified electrode based on the immobilization of Pt particles is fabricated and exhibits electrocatalytic oxidation for L-cysteine (L-Cys), glutathione (GSH) and penicillamine (PEN) with relatively high sensitivity. It is also adaptable to HPLC for pulsed amperometric detection (PAD) of these thiols. PAD largely improves the detection sensitivity because the alternated polarizations can effectively clean and reactivate the electrode surface. It is shown that the peak currents of L-Cys, GSH and PEN are linear to their concentrations, with the calculated detection limit of 1.1 × 10-7, 1.8 × 10-7 and 3.8 × 10-7 mol L-1, respectively (S/N = 3). The method has been successfully applied to assess the contents of L-Cys and GSH in rat striatal microdialysates. The average contents of the two analytes in rat striatum are 2.6 × 10-6 and 2.8 × 10-6 mol L-1, respectively.

AB - A new chemically modified electrode based on the immobilization of Pt particles is fabricated and exhibits electrocatalytic oxidation for L-cysteine (L-Cys), glutathione (GSH) and penicillamine (PEN) with relatively high sensitivity. It is also adaptable to HPLC for pulsed amperometric detection (PAD) of these thiols. PAD largely improves the detection sensitivity because the alternated polarizations can effectively clean and reactivate the electrode surface. It is shown that the peak currents of L-Cys, GSH and PEN are linear to their concentrations, with the calculated detection limit of 1.1 × 10-7, 1.8 × 10-7 and 3.8 × 10-7 mol L-1, respectively (S/N = 3). The method has been successfully applied to assess the contents of L-Cys and GSH in rat striatal microdialysates. The average contents of the two analytes in rat striatum are 2.6 × 10-6 and 2.8 × 10-6 mol L-1, respectively.

KW - Glutathione

KW - L-cysteine

KW - Penicillamine

KW - Pt particles

KW - Pulsed amperometric detection

KW - Striatum

UR - https://www.scopus.com/pages/publications/5044235307

U2 - 10.1002/bmc.357

DO - 10.1002/bmc.357

M3 - 文章

C2 - 15386515

AN - SCOPUS:5044235307

SN - 0269-3879

VL - 18

SP - 564

EP - 569

JO - Biomedical Chromatography

JF - Biomedical Chromatography

IS - 8

ER -

Platinum particles-modified electrode for HPLC with pulsed amperometric detection of thiols in rat striatum

Abstract

Keywords

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