Plasmid DNA cleavage by aminoalkylnaphthalimide-intercalator containing amino groups

Yuanshe Sun; Zhigang Li; Qing Yang; Xuhong Qian

Plasmid DNA cleavage by aminoalkylnaphthalimide-intercalator containing amino groups

Yuanshe Sun^*
, Zhigang Li
, Qing Yang
, Xuhong Qian

^*Corresponding author for this work

Dalian University of Technology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A series of aminoalkylnaphthalimides were synthesized in order to develop novel DNA cleavers. Agarose gel electrophoresis showed supercoiled plasmid DNA with various sizes could be efficiently cleaved in the presence of 100 μmol/L of aminoalkylnaphthalimides at 70°C, pH 7.5. Interestingly, all of cleaved products are close to half-sized DNA fragments in a linear form.

Original language	English
Pages (from-to)	767-771
Number of pages	5
Journal	Chemistry Bulletin / Huaxue Tongbao
Volume	69
Issue number	10
State	Published - 2006
Externally published	Yes

Keywords

Artificial nuclease
DNA intercalator
Naphthalimide
Plasmid DNA

Cite this

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title = "Plasmid DNA cleavage by aminoalkylnaphthalimide-intercalator containing amino groups",

abstract = "A series of aminoalkylnaphthalimides were synthesized in order to develop novel DNA cleavers. Agarose gel electrophoresis showed supercoiled plasmid DNA with various sizes could be efficiently cleaved in the presence of 100 μmol/L of aminoalkylnaphthalimides at 70°C, pH 7.5. Interestingly, all of cleaved products are close to half-sized DNA fragments in a linear form.",

keywords = "Artificial nuclease, DNA intercalator, Naphthalimide, Plasmid DNA",

author = "Yuanshe Sun and Zhigang Li and Qing Yang and Xuhong Qian",

year = "2006",

language = "英语",

volume = "69",

pages = "767--771",

journal = "Chemistry Bulletin / Huaxue Tongbao",

issn = "0441-3776",

publisher = "Editorial Office of Huaxue Tongbao",

number = "10",

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TY - JOUR

T1 - Plasmid DNA cleavage by aminoalkylnaphthalimide-intercalator containing amino groups

AU - Sun, Yuanshe

AU - Li, Zhigang

AU - Yang, Qing

AU - Qian, Xuhong

PY - 2006

Y1 - 2006

N2 - A series of aminoalkylnaphthalimides were synthesized in order to develop novel DNA cleavers. Agarose gel electrophoresis showed supercoiled plasmid DNA with various sizes could be efficiently cleaved in the presence of 100 μmol/L of aminoalkylnaphthalimides at 70°C, pH 7.5. Interestingly, all of cleaved products are close to half-sized DNA fragments in a linear form.

AB - A series of aminoalkylnaphthalimides were synthesized in order to develop novel DNA cleavers. Agarose gel electrophoresis showed supercoiled plasmid DNA with various sizes could be efficiently cleaved in the presence of 100 μmol/L of aminoalkylnaphthalimides at 70°C, pH 7.5. Interestingly, all of cleaved products are close to half-sized DNA fragments in a linear form.

KW - Artificial nuclease

KW - DNA intercalator

KW - Naphthalimide

KW - Plasmid DNA

UR - https://www.scopus.com/pages/publications/33947243560

M3 - 文章

AN - SCOPUS:33947243560

SN - 0441-3776

VL - 69

SP - 767

EP - 771

JO - Chemistry Bulletin / Huaxue Tongbao

JF - Chemistry Bulletin / Huaxue Tongbao

IS - 10

ER -

Plasmid DNA cleavage by aminoalkylnaphthalimide-intercalator containing amino groups

Abstract

Keywords

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