Pim-3 is expressed in endothelial cells and promotes vascular tube formation

Peng Zhang; Hanqin Wang; Xinwen Min; Yinfang Wang; Jie Tang; Jishun Cheng; Dongfeng Li; Xin Chen; Fanjun Cheng; Nanping Wang; Handong Yang

doi:10.1002/jcp.21733

Pim-3 is expressed in endothelial cells and promotes vascular tube formation

Peng Zhang^*, Hanqin Wang, Xinwen Min, Yinfang Wang, Jie Tang, Jishun Cheng, Dongfeng Li, Xin Chen, Fanjun Cheng, Nanping Wang, Handong Yang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Pim-3 is a member of proto-oncogene Pim family that encodes serine/threonine kinases. Pim proteins regulate both apoptosis and cellular metabolism by phosphorylating their substrates. Here, we report for the first time that Pim-3 is highly expressed at mRNA and protein levels in endothelial cells (ECs). We found that Pim-3 is concentrated at the cellular lamellipodia and co-localized with focal adhesion kinase (FAK). Pim-3 was dispersed from lamellipodia when ECs were treated with cytochalasin D, an inhibitor of actin polymerization. In addition, small-interfering RNA (siRNA)-mediated gene knockdown of Pim-3 significantly impaired EC spreading, migration, and proliferation, leading to a reduction in tube-like structure formation in a Matrigel assay. These results provide the novel evidence that Pim-3 plays an essential role in EC spreading and migration, suggesting that Pim-3 may be an important molecular target for the development of small-molecule inhibitors of angiogenesis.

Original language	English
Pages (from-to)	82-90
Number of pages	9
Journal	Journal of Cellular Physiology
Volume	220
Issue number	1
DOIs	https://doi.org/10.1002/jcp.21733
State	Published - Jul 2009
Externally published	Yes

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title = "Pim-3 is expressed in endothelial cells and promotes vascular tube formation",

abstract = "Pim-3 is a member of proto-oncogene Pim family that encodes serine/threonine kinases. Pim proteins regulate both apoptosis and cellular metabolism by phosphorylating their substrates. Here, we report for the first time that Pim-3 is highly expressed at mRNA and protein levels in endothelial cells (ECs). We found that Pim-3 is concentrated at the cellular lamellipodia and co-localized with focal adhesion kinase (FAK). Pim-3 was dispersed from lamellipodia when ECs were treated with cytochalasin D, an inhibitor of actin polymerization. In addition, small-interfering RNA (siRNA)-mediated gene knockdown of Pim-3 significantly impaired EC spreading, migration, and proliferation, leading to a reduction in tube-like structure formation in a Matrigel assay. These results provide the novel evidence that Pim-3 plays an essential role in EC spreading and migration, suggesting that Pim-3 may be an important molecular target for the development of small-molecule inhibitors of angiogenesis.",

author = "Peng Zhang and Hanqin Wang and Xinwen Min and Yinfang Wang and Jie Tang and Jishun Cheng and Dongfeng Li and Xin Chen and Fanjun Cheng and Nanping Wang and Handong Yang",

year = "2009",

month = jul,

doi = "10.1002/jcp.21733",

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pages = "82--90",

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T1 - Pim-3 is expressed in endothelial cells and promotes vascular tube formation

AU - Zhang, Peng

AU - Wang, Hanqin

AU - Min, Xinwen

AU - Wang, Yinfang

AU - Tang, Jie

AU - Cheng, Jishun

AU - Li, Dongfeng

AU - Chen, Xin

AU - Cheng, Fanjun

AU - Wang, Nanping

AU - Yang, Handong

PY - 2009/7

Y1 - 2009/7

N2 - Pim-3 is a member of proto-oncogene Pim family that encodes serine/threonine kinases. Pim proteins regulate both apoptosis and cellular metabolism by phosphorylating their substrates. Here, we report for the first time that Pim-3 is highly expressed at mRNA and protein levels in endothelial cells (ECs). We found that Pim-3 is concentrated at the cellular lamellipodia and co-localized with focal adhesion kinase (FAK). Pim-3 was dispersed from lamellipodia when ECs were treated with cytochalasin D, an inhibitor of actin polymerization. In addition, small-interfering RNA (siRNA)-mediated gene knockdown of Pim-3 significantly impaired EC spreading, migration, and proliferation, leading to a reduction in tube-like structure formation in a Matrigel assay. These results provide the novel evidence that Pim-3 plays an essential role in EC spreading and migration, suggesting that Pim-3 may be an important molecular target for the development of small-molecule inhibitors of angiogenesis.

AB - Pim-3 is a member of proto-oncogene Pim family that encodes serine/threonine kinases. Pim proteins regulate both apoptosis and cellular metabolism by phosphorylating their substrates. Here, we report for the first time that Pim-3 is highly expressed at mRNA and protein levels in endothelial cells (ECs). We found that Pim-3 is concentrated at the cellular lamellipodia and co-localized with focal adhesion kinase (FAK). Pim-3 was dispersed from lamellipodia when ECs were treated with cytochalasin D, an inhibitor of actin polymerization. In addition, small-interfering RNA (siRNA)-mediated gene knockdown of Pim-3 significantly impaired EC spreading, migration, and proliferation, leading to a reduction in tube-like structure formation in a Matrigel assay. These results provide the novel evidence that Pim-3 plays an essential role in EC spreading and migration, suggesting that Pim-3 may be an important molecular target for the development of small-molecule inhibitors of angiogenesis.

UR - https://www.scopus.com/pages/publications/65549111109

U2 - 10.1002/jcp.21733

DO - 10.1002/jcp.21733

M3 - 文章

C2 - 19229879

AN - SCOPUS:65549111109

SN - 0021-9541

VL - 220

SP - 82

EP - 90

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

IS - 1

ER -

Pim-3 is expressed in endothelial cells and promotes vascular tube formation

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