Abstract
We reported a novel femtosecond-laser-activated fluorescence emission from indole solutions upon excitation by the second harmonic wavelength of a femtosecond oscillator. A new absorption band around 400 nm and corresponding fluorescent band in the green domain were produced after the irradiation of femtosecond laser. This femtosecond-laser-activated luminescence process that allows the use of visible wavelength as a substitute for UV light to excite fluorescence from indole would extend applications based on indole chromophore. Furthermore, the photoactived emission can act as a fluorescence lifetime probe to measure the polarity in complex biological systems since it is polaritysensitive. High performance liquid chromatography with fluorescence detector (HPLC-FLU) and high performance liquid chromatography with mass spectrometer (HPLC-MS) analysis demonstrate that the origin of the photoactivated fluorescence is new molecular species that generated in indole solution upon femtosecond laser irradiation.
| Original language | English |
|---|---|
| Pages (from-to) | 2185-2191 |
| Number of pages | 7 |
| Journal | Journal of Fluorescence |
| Volume | 21 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 2011 |
| Externally published | Yes |
Keywords
- Absorption
- Chromatohraphy
- Femtosecond laser
- Fluorescence lifetime
- Indole
- Photoactived fluorescence