TY - JOUR
T1 - PET/d-PET (PdP) Pairing for the Design of Dual-Channel Probes
AU - Guo, Yinghua
AU - Yin, Lei
AU - Qian, Xuhong
AU - Yang, Youjun
AU - Luo, Xiao
N1 - Publisher Copyright:
© 2023 American Chemical Society.
PY - 2023/6/27
Y1 - 2023/6/27
N2 - Design principles of two-channel fluorescence probes are limited. Herein, we report a new principle, i.e., PET/d-PET (PdP) pairing, for the rational design of two-channel probes. Two fluorophores are required in such a PdP-type probe. They mutually quench their fluorescence via PET and d-PET. In the presence of an analyte-of-interest, such a PdP pair is converted into a FRET pair for signaling. The embodiment of such a principle is Rh-TROX, by tethering a rhodamine fluorophore with an ROS-sensitive probe (TotalROX). Fluorescence of both fluorophores in Rh-TROX was quenched as expected. The addition of highly reactive oxidative species led to the recovery of the fluorescence properties of both. The simultaneous fluorescence enhancement in two channels is a viable way to avoid false-positive signals. The new PdP principle could potentially be applied to the development of probes for another range of substrates.
AB - Design principles of two-channel fluorescence probes are limited. Herein, we report a new principle, i.e., PET/d-PET (PdP) pairing, for the rational design of two-channel probes. Two fluorophores are required in such a PdP-type probe. They mutually quench their fluorescence via PET and d-PET. In the presence of an analyte-of-interest, such a PdP pair is converted into a FRET pair for signaling. The embodiment of such a principle is Rh-TROX, by tethering a rhodamine fluorophore with an ROS-sensitive probe (TotalROX). Fluorescence of both fluorophores in Rh-TROX was quenched as expected. The addition of highly reactive oxidative species led to the recovery of the fluorescence properties of both. The simultaneous fluorescence enhancement in two channels is a viable way to avoid false-positive signals. The new PdP principle could potentially be applied to the development of probes for another range of substrates.
UR - https://www.scopus.com/pages/publications/85163786718
U2 - 10.1021/acs.analchem.3c02054
DO - 10.1021/acs.analchem.3c02054
M3 - 文章
AN - SCOPUS:85163786718
SN - 0003-2700
VL - 95
SP - 9722
EP - 9728
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 25
ER -