TY - JOUR
T1 - Overexpression of the Arabidopsis gene UPRIGHT ROSETTE reveals a homeostatic control for indole-3-acetic acid
AU - Sun, Yue
AU - Yang, Yang
AU - Yuan, Zheng
AU - Muller, Jutta Ludwig
AU - Yu, Chen
AU - Xu, Yanfei
AU - Shao, Xinghua
AU - Li, Xiaofang
AU - Decker, Eva L.
AU - Reski, Ralf
AU - Huang, Hai
PY - 2010/7
Y1 - 2010/7
N2 - Auxins are phytohormones that are essential for many aspects of plant growth and development. The main auxin produced by plants is indole-3-acetic acid (IAA). IAA exists in free and conjugated forms, corresponding to the bioactive and stored hormones, respectively. Free IAA levels, which are crucial for various physiological activities, are maintained through a complex network of environmentally and developmentally responsive pathways including IAA biosynthesis, transport, degradation, conjugation, and conjugate hydrolysis. Among conjugated IAA forms, ester and amide-type conjugates are the most common. Here we identify a new gene, UPRIGHT ROSETTE (URO), the overexpression of which alters IAA homeostasis in Arabidopsis (Arabidopsis thaliana). We previously identified a semidominant mutant, uro, which had multiple auxin-related phenotypes. We show here that compared to wild-type plants, the uro plants contain increased levels of free and esterconjugated IAA, and decreased levels of amino-conjugated IAA. uro plants carrying the pDR5:b-glucuronidase (GUS) construct have strong GUS staining in cotyledons and stem, and their cotyledons are able to generate roots on auxin-free medium, further confirming that this mutant contains higher levels of free IAA. The URO gene encodes a C2H2 zinc-finger protein that belongs to a plant-specific gene family. The response to URO overexpression is evolutionarily conserved among plants, as GUS activity that may reflect free IAA levels was increased markedly in transgenic p35S:URO/pGH3:GUS/Physcomitrella patens and pNOS:URO/pGH3:GUS/P. patens plants.
AB - Auxins are phytohormones that are essential for many aspects of plant growth and development. The main auxin produced by plants is indole-3-acetic acid (IAA). IAA exists in free and conjugated forms, corresponding to the bioactive and stored hormones, respectively. Free IAA levels, which are crucial for various physiological activities, are maintained through a complex network of environmentally and developmentally responsive pathways including IAA biosynthesis, transport, degradation, conjugation, and conjugate hydrolysis. Among conjugated IAA forms, ester and amide-type conjugates are the most common. Here we identify a new gene, UPRIGHT ROSETTE (URO), the overexpression of which alters IAA homeostasis in Arabidopsis (Arabidopsis thaliana). We previously identified a semidominant mutant, uro, which had multiple auxin-related phenotypes. We show here that compared to wild-type plants, the uro plants contain increased levels of free and esterconjugated IAA, and decreased levels of amino-conjugated IAA. uro plants carrying the pDR5:b-glucuronidase (GUS) construct have strong GUS staining in cotyledons and stem, and their cotyledons are able to generate roots on auxin-free medium, further confirming that this mutant contains higher levels of free IAA. The URO gene encodes a C2H2 zinc-finger protein that belongs to a plant-specific gene family. The response to URO overexpression is evolutionarily conserved among plants, as GUS activity that may reflect free IAA levels was increased markedly in transgenic p35S:URO/pGH3:GUS/Physcomitrella patens and pNOS:URO/pGH3:GUS/P. patens plants.
UR - https://www.scopus.com/pages/publications/77954323795
U2 - 10.1104/pp.110.154021
DO - 10.1104/pp.110.154021
M3 - 文章
C2 - 20466845
AN - SCOPUS:77954323795
SN - 0032-0889
VL - 153
SP - 1311
EP - 1320
JO - Plant Physiology
JF - Plant Physiology
IS - 3
ER -