One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing

Cuiling Zhang; Caiping Ding; Guohua Zhou; Qin Xue; Yuezhong Xian

doi:10.1016/j.aca.2016.12.024

One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing

Cuiling Zhang
, Caiping Ding
, Guohua Zhou
, Qin Xue
, Yuezhong Xian^*

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn²⁺) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS₂ nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn²⁺ QDs, but also provides a platform for the investigation of small molecule-protein interaction.

Original language	English
Pages (from-to)	63-69
Number of pages	7
Journal	Analytica Chimica Acta
Volume	957
DOIs	https://doi.org/10.1016/j.aca.2016.12.024
State	Published - 8 Mar 2017

Keywords

Biosensor
FRET
Quantum dots
Terminal protection of small-molecule linked DNA
WS

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10.1016/j.aca.2016.12.024

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title = "One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing",

abstract = "DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn2+) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn2+ QDs, but also provides a platform for the investigation of small molecule-protein interaction.",

keywords = "Biosensor, FRET, Quantum dots, Terminal protection of small-molecule linked DNA, WS",

author = "Cuiling Zhang and Caiping Ding and Guohua Zhou and Qin Xue and Yuezhong Xian",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2017",

month = mar,

day = "8",

doi = "10.1016/j.aca.2016.12.024",

language = "英语",

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TY - JOUR

T1 - One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing

AU - Zhang, Cuiling

AU - Ding, Caiping

AU - Zhou, Guohua

AU - Xue, Qin

AU - Xian, Yuezhong

PY - 2017/3/8

Y1 - 2017/3/8

N2 - DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn2+) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn2+ QDs, but also provides a platform for the investigation of small molecule-protein interaction.

AB - DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn2+) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn2+ QDs, but also provides a platform for the investigation of small molecule-protein interaction.

KW - Biosensor

KW - FRET

KW - Quantum dots

KW - Terminal protection of small-molecule linked DNA

KW - WS

UR - https://www.scopus.com/pages/publications/85009371987

U2 - 10.1016/j.aca.2016.12.024

DO - 10.1016/j.aca.2016.12.024

M3 - 文章

C2 - 28107835

AN - SCOPUS:85009371987

SN - 0003-2670

VL - 957

SP - 63

EP - 69

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

ER -

One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing

Abstract

Keywords

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