TY - JOUR
T1 - Novel nitric oxide microsensor and its application to the study of smooth muscle cells
AU - Zhu, Min
AU - Liu, Min
AU - Shi, Guoyue
AU - Xu, Fang
AU - Ye, Xiyun
AU - Chen, Junshui
AU - Jin, Litong
AU - Jin, Jiye
PY - 2002/3/25
Y1 - 2002/3/25
N2 - A novel sensitive, selective and stable nitric oxide (NO) microsensor is described, which is modified by nano Au colloid and Nafion. As determined by atomic forced microscopy (AFM), the diameter of Au colloid particles is from 7 to 14nm. The detection of NO is based on the nano Au particles catalysis of NO oxidation at an anodic potential of +0.74V (versus saturated calomel electrode (SCE)). The microsensor showed a low detection limit, high selectivity and sensitivity for NO determination. The oxidation current (measured by differential pulse amperometric technique) was linear with NO concentration ranging from 1.0×10-7 to 4.0×10-5mol/l with a calculated detection limit of 5.0×10-8mol/l (S/N=3). Using the microsensor, the direct real time production of NO in the smooth muscle cells was continuously measured, which showed the NO levels was increased by stimulating with L-arginine (L-Arg), acetylcholine (Ach) and a self-made flavonoid medicine.
AB - A novel sensitive, selective and stable nitric oxide (NO) microsensor is described, which is modified by nano Au colloid and Nafion. As determined by atomic forced microscopy (AFM), the diameter of Au colloid particles is from 7 to 14nm. The detection of NO is based on the nano Au particles catalysis of NO oxidation at an anodic potential of +0.74V (versus saturated calomel electrode (SCE)). The microsensor showed a low detection limit, high selectivity and sensitivity for NO determination. The oxidation current (measured by differential pulse amperometric technique) was linear with NO concentration ranging from 1.0×10-7 to 4.0×10-5mol/l with a calculated detection limit of 5.0×10-8mol/l (S/N=3). Using the microsensor, the direct real time production of NO in the smooth muscle cells was continuously measured, which showed the NO levels was increased by stimulating with L-arginine (L-Arg), acetylcholine (Ach) and a self-made flavonoid medicine.
KW - Au colloid modified microsensor
KW - Nitric oxide
KW - Smooth muscle cells
UR - https://www.scopus.com/pages/publications/0037170668
U2 - 10.1016/S0003-2670(01)01599-9
DO - 10.1016/S0003-2670(01)01599-9
M3 - 文章
AN - SCOPUS:0037170668
SN - 0003-2670
VL - 455
SP - 199
EP - 206
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 2
ER -