Novel conducting polymers for DNA sensing

Hui Peng; Christian Soeller; Jadranka Travas-Sejdic

doi:10.1021/ma062060g

Novel conducting polymers for DNA sensing

Hui Peng
, Christian Soeller
, Jadranka Travas-Sejdic^*

^*Corresponding author for this work

The University of Auckland

Research output: Contribution to journal › Article › peer-review

112 Scopus citations

Abstract

Novel functionalized pyrroles, 3-pyrrolylacrylic acid (PAA), 5-(3-pyrrolyl) 2,4-pentadienoic acid (PPDA), and 3-pyrrolylpentanoic acid (PPA) were synthesized and used to construct label-free gene sensors based on pyrrole copolymer films. Electrochemical impedance spectroscopy (EIS) was used to obtain electrical readout from these gene sensors. Comparison of the performance of PPDA-containing sensors to that containing 3-pyrrolylpentanoic acid (PPA), a similar functionalized pyrrole but with a saturated side chain, showed that functionalized polymers with unsaturated side chains have superior properties for use in biosensor applications. Sensors based on copolymers of both PAA and PPDA were evaluated across a wide range of oligonucleotide concentrations. The highest sensitivity was exhibited by a poly(Py-co-PPDA) sensor whose EIS spectra were well resolved, and the changes in charge-transfer resistance, taken as the index of sensor response, were largest among the materials studied. This sensor had a detection limit of 0.5 nM and a good selectivity.

Original language	English
Pages (from-to)	909-914
Number of pages	6
Journal	Macromolecules
Volume	40
Issue number	4
DOIs	https://doi.org/10.1021/ma062060g
State	Published - 20 Feb 2007
Externally published	Yes

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title = "Novel conducting polymers for DNA sensing",

abstract = "Novel functionalized pyrroles, 3-pyrrolylacrylic acid (PAA), 5-(3-pyrrolyl) 2,4-pentadienoic acid (PPDA), and 3-pyrrolylpentanoic acid (PPA) were synthesized and used to construct label-free gene sensors based on pyrrole copolymer films. Electrochemical impedance spectroscopy (EIS) was used to obtain electrical readout from these gene sensors. Comparison of the performance of PPDA-containing sensors to that containing 3-pyrrolylpentanoic acid (PPA), a similar functionalized pyrrole but with a saturated side chain, showed that functionalized polymers with unsaturated side chains have superior properties for use in biosensor applications. Sensors based on copolymers of both PAA and PPDA were evaluated across a wide range of oligonucleotide concentrations. The highest sensitivity was exhibited by a poly(Py-co-PPDA) sensor whose EIS spectra were well resolved, and the changes in charge-transfer resistance, taken as the index of sensor response, were largest among the materials studied. This sensor had a detection limit of 0.5 nM and a good selectivity.",

author = "Hui Peng and Christian Soeller and Jadranka Travas-Sejdic",

year = "2007",

month = feb,

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doi = "10.1021/ma062060g",

language = "英语",

volume = "40",

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journal = "Macromolecules",

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TY - JOUR

T1 - Novel conducting polymers for DNA sensing

AU - Peng, Hui

AU - Soeller, Christian

AU - Travas-Sejdic, Jadranka

PY - 2007/2/20

Y1 - 2007/2/20

N2 - Novel functionalized pyrroles, 3-pyrrolylacrylic acid (PAA), 5-(3-pyrrolyl) 2,4-pentadienoic acid (PPDA), and 3-pyrrolylpentanoic acid (PPA) were synthesized and used to construct label-free gene sensors based on pyrrole copolymer films. Electrochemical impedance spectroscopy (EIS) was used to obtain electrical readout from these gene sensors. Comparison of the performance of PPDA-containing sensors to that containing 3-pyrrolylpentanoic acid (PPA), a similar functionalized pyrrole but with a saturated side chain, showed that functionalized polymers with unsaturated side chains have superior properties for use in biosensor applications. Sensors based on copolymers of both PAA and PPDA were evaluated across a wide range of oligonucleotide concentrations. The highest sensitivity was exhibited by a poly(Py-co-PPDA) sensor whose EIS spectra were well resolved, and the changes in charge-transfer resistance, taken as the index of sensor response, were largest among the materials studied. This sensor had a detection limit of 0.5 nM and a good selectivity.

AB - Novel functionalized pyrroles, 3-pyrrolylacrylic acid (PAA), 5-(3-pyrrolyl) 2,4-pentadienoic acid (PPDA), and 3-pyrrolylpentanoic acid (PPA) were synthesized and used to construct label-free gene sensors based on pyrrole copolymer films. Electrochemical impedance spectroscopy (EIS) was used to obtain electrical readout from these gene sensors. Comparison of the performance of PPDA-containing sensors to that containing 3-pyrrolylpentanoic acid (PPA), a similar functionalized pyrrole but with a saturated side chain, showed that functionalized polymers with unsaturated side chains have superior properties for use in biosensor applications. Sensors based on copolymers of both PAA and PPDA were evaluated across a wide range of oligonucleotide concentrations. The highest sensitivity was exhibited by a poly(Py-co-PPDA) sensor whose EIS spectra were well resolved, and the changes in charge-transfer resistance, taken as the index of sensor response, were largest among the materials studied. This sensor had a detection limit of 0.5 nM and a good selectivity.

UR - https://www.scopus.com/pages/publications/33847740358

U2 - 10.1021/ma062060g

DO - 10.1021/ma062060g

M3 - 文章

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SN - 0024-9297

VL - 40

SP - 909

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JO - Macromolecules

JF - Macromolecules

IS - 4

ER -

Novel conducting polymers for DNA sensing

Abstract

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