Abstract
Glutathione (GSH) plays a critical role in maintaining cellular redox homeostasis in biological system. Mitochondrion is a pivotal organelle for cellular aerobic respiration and its disorder is associated with impaired redox balance, leading to cell death. In this work, we designed and synthesized a non-invasive “off-on” mitochondrial-targeting fluorescent probe QZ for the detection of GSH in living cells. Based on the mechanism of native chemical ligation (NCL) and fluorescence resonance energy transfer (FRET), a rhodamine B derivative, QZ was prepared, by choosing aromatic thioester bond as the selective reaction site. QZ exhibited excellent detection capability for GSH over Cys and Hcy. Upon addition of GSH to QZ solution, a remarkably enhanced fluorescence was observed with a limit of detection of 2.98 µmol/L. Furthermore, QZ was found to possess the specific mitochondrial localization ability in cell imaging experiments. Moreover, with exogenous and endogenous stimulations, QZ could image GSH in living cells.
| Original language | English |
|---|---|
| Pages (from-to) | 3899-3902 |
| Number of pages | 4 |
| Journal | Chinese Chemical Letters |
| Volume | 32 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2021 |
| Externally published | Yes |
Keywords
- Fluorescent probe
- Glutathione
- In vivo imaging
- NCL