mRNA expression profiling reveals a role of Helicobacter pylori vacuolating toxin in escaping host defense

Jian Ping Yuan; Tao Li; Zhen Hong Li; Gui Zhen Yang; Bao Yu Hu; Xiao Dong Shi; Tie Liu Shi; Shan Qing Tong; Xiao Kui Guo

doi:10.3748/wjg.v10.i10.1528

mRNA expression profiling reveals a role of Helicobacter pylori vacuolating toxin in escaping host defense

Jian Ping Yuan, Tao Li, Zhen Hong Li, Gui Zhen Yang, Bao Yu Hu, Xiao Dong Shi, Tie Liu Shi, Shan Qing Tong, Xiao Kui Guo

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Aim: To study the immune response of host to Helicobacter pylori VacA. Methods: The monocyte/macrophage-like U937 cells were infected with Helicobacter pylori vacA-positive strain NCTC 11638 or isogenic vacA-negative mutant. Differentially expressed genes were identified at 2, 6, 10, and 24 h post-infection by cDNA microarray. Differential expressions of some genes were confirmed by Northern blot. Results: More than 100 genes altered their mRNA expression at different time points respectively, many of which were identified to be related to immune evasion. Conclusion: VacA is a crucial element for H pylori to escape from host immune defense by means of differentially regulating the expression of some related genes. These genes, previously known or unknown to be involved in the mechanism of immune evasion, deserve further investigation to unearth much more information complicated in the immune response.

Original language	English
Pages (from-to)	1528-1532
Number of pages	5
Journal	World Journal of Gastroenterology
Volume	10
Issue number	10
DOIs	https://doi.org/10.3748/wjg.v10.i10.1528
State	Published - 15 May 2004
Externally published	Yes

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title = "mRNA expression profiling reveals a role of Helicobacter pylori vacuolating toxin in escaping host defense",

abstract = "Aim: To study the immune response of host to Helicobacter pylori VacA. Methods: The monocyte/macrophage-like U937 cells were infected with Helicobacter pylori vacA-positive strain NCTC 11638 or isogenic vacA-negative mutant. Differentially expressed genes were identified at 2, 6, 10, and 24 h post-infection by cDNA microarray. Differential expressions of some genes were confirmed by Northern blot. Results: More than 100 genes altered their mRNA expression at different time points respectively, many of which were identified to be related to immune evasion. Conclusion: VacA is a crucial element for H pylori to escape from host immune defense by means of differentially regulating the expression of some related genes. These genes, previously known or unknown to be involved in the mechanism of immune evasion, deserve further investigation to unearth much more information complicated in the immune response.",

author = "Yuan, \{Jian Ping\} and Tao Li and Li, \{Zhen Hong\} and Yang, \{Gui Zhen\} and Hu, \{Bao Yu\} and Shi, \{Xiao Dong\} and Shi, \{Tie Liu\} and Tong, \{Shan Qing\} and Guo, \{Xiao Kui\}",

year = "2004",

month = may,

day = "15",

doi = "10.3748/wjg.v10.i10.1528",

language = "英语",

volume = "10",

pages = "1528--1532",

journal = "World Journal of Gastroenterology",

issn = "1007-9327",

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number = "10",

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TY - JOUR

T1 - mRNA expression profiling reveals a role of Helicobacter pylori vacuolating toxin in escaping host defense

AU - Yuan, Jian Ping

AU - Li, Tao

AU - Li, Zhen Hong

AU - Yang, Gui Zhen

AU - Hu, Bao Yu

AU - Shi, Xiao Dong

AU - Shi, Tie Liu

AU - Tong, Shan Qing

AU - Guo, Xiao Kui

PY - 2004/5/15

Y1 - 2004/5/15

N2 - Aim: To study the immune response of host to Helicobacter pylori VacA. Methods: The monocyte/macrophage-like U937 cells were infected with Helicobacter pylori vacA-positive strain NCTC 11638 or isogenic vacA-negative mutant. Differentially expressed genes were identified at 2, 6, 10, and 24 h post-infection by cDNA microarray. Differential expressions of some genes were confirmed by Northern blot. Results: More than 100 genes altered their mRNA expression at different time points respectively, many of which were identified to be related to immune evasion. Conclusion: VacA is a crucial element for H pylori to escape from host immune defense by means of differentially regulating the expression of some related genes. These genes, previously known or unknown to be involved in the mechanism of immune evasion, deserve further investigation to unearth much more information complicated in the immune response.

AB - Aim: To study the immune response of host to Helicobacter pylori VacA. Methods: The monocyte/macrophage-like U937 cells were infected with Helicobacter pylori vacA-positive strain NCTC 11638 or isogenic vacA-negative mutant. Differentially expressed genes were identified at 2, 6, 10, and 24 h post-infection by cDNA microarray. Differential expressions of some genes were confirmed by Northern blot. Results: More than 100 genes altered their mRNA expression at different time points respectively, many of which were identified to be related to immune evasion. Conclusion: VacA is a crucial element for H pylori to escape from host immune defense by means of differentially regulating the expression of some related genes. These genes, previously known or unknown to be involved in the mechanism of immune evasion, deserve further investigation to unearth much more information complicated in the immune response.

UR - https://www.scopus.com/pages/publications/4444262260

U2 - 10.3748/wjg.v10.i10.1528

DO - 10.3748/wjg.v10.i10.1528

M3 - 文章

C2 - 15133867

AN - SCOPUS:4444262260

SN - 1007-9327

VL - 10

SP - 1528

EP - 1532

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

IS - 10

ER -

mRNA expression profiling reveals a role of Helicobacter pylori vacuolating toxin in escaping host defense

Abstract

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