TY - JOUR
T1 - Monitoring of endogenous hydrogen sulfide in living cells using surface-enhanced Raman scattering
AU - Li, Da Wei
AU - Qu, Lu Lu
AU - Hu, Kai
AU - Long, Yi Tao
AU - Tian, He
N1 - Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - Hydrogen sulfide (H2S) has emerged as an important gasotransmitter in diverse physiological processes, although many aspects of its roles remain unclear, partly owing to a lack of robust analytical methods. Herein we report a novel surface-enhanced Raman scattering (SERS) nanosensor, 4-acetamidobenzenesulfonyl azide-functionalized gold nanoparticles (AuNPs/4-AA), for detecting the endogenous H2S in living cells. The detection is accomplished with SERS spectrum changes of AuNPs/4-AA resulting from the reaction of H2S with 4-AA on AuNPs. The SERS nanosensor exhibits high selectivity toward H2S. Furthermore, AuNPs/4-AA responds to H2S within 1 min with a 0.1 μM level of sensitivity. In particular, our SERS method can be utilized to monitor the endogenous H2S generated in living glioma cells, demonstrating its great promise in studies of pathophysiological pathways involving H2S. Rapid, selective, and sensitive: The endogenous H2S in living cells can be detected rapidly, selectively, and sensitively using a surface-enhanced Raman scattering (SERS) nanosensor, 4-acetamidobenzenesulfonyl azide-functionalized gold nanoparticles (AuNPs/4-AA). Based on the rapid and specific reaction between H2S and 4-AA, combined with the sensitive fingerprinting capability of SERS, the nanosensor can monitor the endogenous H2S generated in a variety of pathophysiological pathways.
AB - Hydrogen sulfide (H2S) has emerged as an important gasotransmitter in diverse physiological processes, although many aspects of its roles remain unclear, partly owing to a lack of robust analytical methods. Herein we report a novel surface-enhanced Raman scattering (SERS) nanosensor, 4-acetamidobenzenesulfonyl azide-functionalized gold nanoparticles (AuNPs/4-AA), for detecting the endogenous H2S in living cells. The detection is accomplished with SERS spectrum changes of AuNPs/4-AA resulting from the reaction of H2S with 4-AA on AuNPs. The SERS nanosensor exhibits high selectivity toward H2S. Furthermore, AuNPs/4-AA responds to H2S within 1 min with a 0.1 μM level of sensitivity. In particular, our SERS method can be utilized to monitor the endogenous H2S generated in living glioma cells, demonstrating its great promise in studies of pathophysiological pathways involving H2S. Rapid, selective, and sensitive: The endogenous H2S in living cells can be detected rapidly, selectively, and sensitively using a surface-enhanced Raman scattering (SERS) nanosensor, 4-acetamidobenzenesulfonyl azide-functionalized gold nanoparticles (AuNPs/4-AA). Based on the rapid and specific reaction between H2S and 4-AA, combined with the sensitive fingerprinting capability of SERS, the nanosensor can monitor the endogenous H2S generated in a variety of pathophysiological pathways.
KW - hydrogen sulfide
KW - living cells
KW - monitoring
KW - nanosensors
KW - surface-enhanced Raman spectroscopy
UR - https://www.scopus.com/pages/publications/84944276797
U2 - 10.1002/anie.201505025
DO - 10.1002/anie.201505025
M3 - 文章
C2 - 26314839
AN - SCOPUS:84944276797
SN - 1433-7851
VL - 54
SP - 12758
EP - 12761
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 43
ER -