Molecular characterization of RIG-I, STAT-1 and IFN-beta in the horseshoe bat

Jinju Li; Guangxu Zhang; Dalong Cheng; Hua Ren; Min Qian; Bing Du

doi:10.1016/j.gene.2015.02.013

Molecular characterization of RIG-I, STAT-1 and IFN-beta in the horseshoe bat

Jinju Li, Guangxu Zhang, Dalong Cheng, Hua Ren, Min Qian, Bing Du

School of Life Sciences

East China Normal University

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Wild Chinese horseshoe bats have been proven to be natural reservoirs of SARS-like coronaviruses. However, the molecular characterization of key proteins in bats still needs to be explored further. In this study, we used cloning and bioinformatics to analyze the sequence of RIG-I, STAT-1 and IFN-β in the immortalized cell lines from Rhinolophus affinis and Rhinolophus sinicus. Then, we treated different bat cells, mouse embryonic fibroblasts (MEF) and splenocytes with polyinosinic-polycytidylic acid (polyI:C) and vesicular stomatitis virus (VSV) to assess and compare antiviral immune responses between bats and mice. Our results demonstrated that bat RIG-I, STAT-1 and IFN-β showed close homology with human, mouse, pig and rhesus monkey. RIG-I and STAT-1 were both highly expressed in bat spleen. Furthermore, IFN-β was induced by polyI:C and VSV in both bat and mouse cells. These findings have provided new insight into the potential characteristics of the bat innate immune system against viral infection.

Original language	English
Pages (from-to)	115-123
Number of pages	9
Journal	Gene
Volume	561
Issue number	1
DOIs	https://doi.org/10.1016/j.gene.2015.02.013
State	Published - 25 Apr 2015

Keywords

Bat
IFN-β
RIG-I
STAT-1
VSV

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title = "Molecular characterization of RIG-I, STAT-1 and IFN-beta in the horseshoe bat",

abstract = "Wild Chinese horseshoe bats have been proven to be natural reservoirs of SARS-like coronaviruses. However, the molecular characterization of key proteins in bats still needs to be explored further. In this study, we used cloning and bioinformatics to analyze the sequence of RIG-I, STAT-1 and IFN-β in the immortalized cell lines from Rhinolophus affinis and Rhinolophus sinicus. Then, we treated different bat cells, mouse embryonic fibroblasts (MEF) and splenocytes with polyinosinic-polycytidylic acid (polyI:C) and vesicular stomatitis virus (VSV) to assess and compare antiviral immune responses between bats and mice. Our results demonstrated that bat RIG-I, STAT-1 and IFN-β showed close homology with human, mouse, pig and rhesus monkey. RIG-I and STAT-1 were both highly expressed in bat spleen. Furthermore, IFN-β was induced by polyI:C and VSV in both bat and mouse cells. These findings have provided new insight into the potential characteristics of the bat innate immune system against viral infection.",

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author = "Jinju Li and Guangxu Zhang and Dalong Cheng and Hua Ren and Min Qian and Bing Du",

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year = "2015",

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doi = "10.1016/j.gene.2015.02.013",

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T1 - Molecular characterization of RIG-I, STAT-1 and IFN-beta in the horseshoe bat

AU - Li, Jinju

AU - Zhang, Guangxu

AU - Cheng, Dalong

AU - Ren, Hua

AU - Qian, Min

AU - Du, Bing

PY - 2015/4/25

Y1 - 2015/4/25

N2 - Wild Chinese horseshoe bats have been proven to be natural reservoirs of SARS-like coronaviruses. However, the molecular characterization of key proteins in bats still needs to be explored further. In this study, we used cloning and bioinformatics to analyze the sequence of RIG-I, STAT-1 and IFN-β in the immortalized cell lines from Rhinolophus affinis and Rhinolophus sinicus. Then, we treated different bat cells, mouse embryonic fibroblasts (MEF) and splenocytes with polyinosinic-polycytidylic acid (polyI:C) and vesicular stomatitis virus (VSV) to assess and compare antiviral immune responses between bats and mice. Our results demonstrated that bat RIG-I, STAT-1 and IFN-β showed close homology with human, mouse, pig and rhesus monkey. RIG-I and STAT-1 were both highly expressed in bat spleen. Furthermore, IFN-β was induced by polyI:C and VSV in both bat and mouse cells. These findings have provided new insight into the potential characteristics of the bat innate immune system against viral infection.

AB - Wild Chinese horseshoe bats have been proven to be natural reservoirs of SARS-like coronaviruses. However, the molecular characterization of key proteins in bats still needs to be explored further. In this study, we used cloning and bioinformatics to analyze the sequence of RIG-I, STAT-1 and IFN-β in the immortalized cell lines from Rhinolophus affinis and Rhinolophus sinicus. Then, we treated different bat cells, mouse embryonic fibroblasts (MEF) and splenocytes with polyinosinic-polycytidylic acid (polyI:C) and vesicular stomatitis virus (VSV) to assess and compare antiviral immune responses between bats and mice. Our results demonstrated that bat RIG-I, STAT-1 and IFN-β showed close homology with human, mouse, pig and rhesus monkey. RIG-I and STAT-1 were both highly expressed in bat spleen. Furthermore, IFN-β was induced by polyI:C and VSV in both bat and mouse cells. These findings have provided new insight into the potential characteristics of the bat innate immune system against viral infection.

KW - Bat

KW - IFN-β

KW - RIG-I

KW - STAT-1

KW - VSV

UR - https://www.scopus.com/pages/publications/84924606065

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DO - 10.1016/j.gene.2015.02.013

M3 - 文章

C2 - 25680291

AN - SCOPUS:84924606065

SN - 0378-1119

VL - 561

SP - 115

EP - 123

JO - Gene

JF - Gene

IS - 1

ER -

Molecular characterization of RIG-I, STAT-1 and IFN-beta in the horseshoe bat

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Keywords

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