"molecular beacon"-hosted thioflavin T: Applications for label-free fluorescent detection of iodide and logic operations

Yan Yun Li; Xiao Qin Jiang; Ling Fei Lu; Min Zhang; Guoyue Shi

doi:10.1016/j.talanta.2016.01.003

"molecular beacon"-hosted thioflavin T: Applications for label-free fluorescent detection of iodide and logic operations

Yan Yun Li
, Xiao Qin Jiang
, Ling Fei Lu
, Min Zhang^*
, Guoyue Shi

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

In this work, we presented a simple, label-free and rapid-responsive fluorescence assay for iodide (I^-) detection based on "molecular beacon (MB)"-hosted thioflavin T (ThT), achieving a limit of detection as low as 158 nM. The proposed method exhibited very good selectivity to I^- ions over other anions interference due to the strong binding force between I^- ions with Hg²⁺. Upon the addition of I^- ions, it would capture Hg²⁺ from a T-Hg²⁺-T complex belonging to the MB-like DNA hairpin structure, which eventually quenched the initial fluorescence as output. In addition, it was successfully applied for operation of an integrated DNA logic gate system and to the determination of I^- in real samples such as human urine.

Original language	English
Pages (from-to)	615-621
Number of pages	7
Journal	Talanta
Volume	150
DOIs	https://doi.org/10.1016/j.talanta.2016.01.003
State	Published - 1 Apr 2016

Keywords

DNA
Iodide
Logic gate
Molecular beacon
Thioflavin T

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10.1016/j.talanta.2016.01.003

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title = "{"}molecular beacon{"}-hosted thioflavin T: Applications for label-free fluorescent detection of iodide and logic operations",

abstract = "In this work, we presented a simple, label-free and rapid-responsive fluorescence assay for iodide (I-) detection based on {"}molecular beacon (MB){"}-hosted thioflavin T (ThT), achieving a limit of detection as low as 158 nM. The proposed method exhibited very good selectivity to I- ions over other anions interference due to the strong binding force between I- ions with Hg2+. Upon the addition of I- ions, it would capture Hg2+ from a T-Hg2+-T complex belonging to the MB-like DNA hairpin structure, which eventually quenched the initial fluorescence as output. In addition, it was successfully applied for operation of an integrated DNA logic gate system and to the determination of I- in real samples such as human urine.",

keywords = "DNA, Iodide, Logic gate, Molecular beacon, Thioflavin T",

author = "Li, \{Yan Yun\} and Jiang, \{Xiao Qin\} and Lu, \{Ling Fei\} and Min Zhang and Guoyue Shi",

year = "2016",

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doi = "10.1016/j.talanta.2016.01.003",

language = "英语",

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journal = "Talanta",

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TY - JOUR

T1 - "molecular beacon"-hosted thioflavin T

T2 - Applications for label-free fluorescent detection of iodide and logic operations

AU - Li, Yan Yun

AU - Jiang, Xiao Qin

AU - Lu, Ling Fei

AU - Zhang, Min

AU - Shi, Guoyue

PY - 2016/4/1

Y1 - 2016/4/1

N2 - In this work, we presented a simple, label-free and rapid-responsive fluorescence assay for iodide (I-) detection based on "molecular beacon (MB)"-hosted thioflavin T (ThT), achieving a limit of detection as low as 158 nM. The proposed method exhibited very good selectivity to I- ions over other anions interference due to the strong binding force between I- ions with Hg2+. Upon the addition of I- ions, it would capture Hg2+ from a T-Hg2+-T complex belonging to the MB-like DNA hairpin structure, which eventually quenched the initial fluorescence as output. In addition, it was successfully applied for operation of an integrated DNA logic gate system and to the determination of I- in real samples such as human urine.

AB - In this work, we presented a simple, label-free and rapid-responsive fluorescence assay for iodide (I-) detection based on "molecular beacon (MB)"-hosted thioflavin T (ThT), achieving a limit of detection as low as 158 nM. The proposed method exhibited very good selectivity to I- ions over other anions interference due to the strong binding force between I- ions with Hg2+. Upon the addition of I- ions, it would capture Hg2+ from a T-Hg2+-T complex belonging to the MB-like DNA hairpin structure, which eventually quenched the initial fluorescence as output. In addition, it was successfully applied for operation of an integrated DNA logic gate system and to the determination of I- in real samples such as human urine.

KW - DNA

KW - Iodide

KW - Logic gate

KW - Molecular beacon

KW - Thioflavin T

UR - https://www.scopus.com/pages/publications/84953923867

U2 - 10.1016/j.talanta.2016.01.003

DO - 10.1016/j.talanta.2016.01.003

M3 - 文章

C2 - 26838450

AN - SCOPUS:84953923867

SN - 0039-9140

VL - 150

SP - 615

EP - 621

JO - Talanta

JF - Talanta

ER -

"molecular beacon"-hosted thioflavin T: Applications for label-free fluorescent detection of iodide and logic operations

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Keywords

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