TY - JOUR
T1 - Metabolic pathway for degradation of anthracene by halophilic Martelella sp. AD-3
AU - Cui, Changzheng
AU - Ma, Lei
AU - Shi, Jie
AU - Lin, Kuangfei
AU - Luo, Qishi
AU - Liu, Yongdi
PY - 2014/4
Y1 - 2014/4
N2 - Anthracene (40mgl-1) was completely depleted by Martelella sp. strain AD-3 under 3% salinity and a pH of 9.0 after 6 days of incubation. The metabolites were extracted and identified by high-performance liquid chromatography (HPLC) retention times, mass spectrometry, 1H and 13C nuclear magnetic resonance spectrometry, and comparison to authentic compounds or literature data. On the basis of the identified metabolites, enzyme activities and the utilization of probable intermediates, anthracene degradation by strain AD-3 is proposed via two distinct routes. In route I, metabolism of anthracene is initiated by the dioxygenation at C-1,2, then proceeds through 6,7-benzocoumarin, 3-hydroxy-2-naphthoic acid, salicylic acid and gentisic acid. In route II, anthracene is metabolized to 9,10-anthraquinone. The results suggest that strain AD-3 possesses efficient anthracene biodegradability in high salinity. To our knowledge, this work presents the first report of anthracene degradation by a halophilic PAH-degrading strain via two routes. The strain AD-3 may be a useful candidate for PAH-contaminated saline-alkali soil bioremediation.
AB - Anthracene (40mgl-1) was completely depleted by Martelella sp. strain AD-3 under 3% salinity and a pH of 9.0 after 6 days of incubation. The metabolites were extracted and identified by high-performance liquid chromatography (HPLC) retention times, mass spectrometry, 1H and 13C nuclear magnetic resonance spectrometry, and comparison to authentic compounds or literature data. On the basis of the identified metabolites, enzyme activities and the utilization of probable intermediates, anthracene degradation by strain AD-3 is proposed via two distinct routes. In route I, metabolism of anthracene is initiated by the dioxygenation at C-1,2, then proceeds through 6,7-benzocoumarin, 3-hydroxy-2-naphthoic acid, salicylic acid and gentisic acid. In route II, anthracene is metabolized to 9,10-anthraquinone. The results suggest that strain AD-3 possesses efficient anthracene biodegradability in high salinity. To our knowledge, this work presents the first report of anthracene degradation by a halophilic PAH-degrading strain via two routes. The strain AD-3 may be a useful candidate for PAH-contaminated saline-alkali soil bioremediation.
KW - 3-Hydroxy-2-naphthoic acid
KW - Anthracene
KW - Biodegradation
KW - Metabolic pathway
KW - Moderate halophilic bacteria
UR - https://www.scopus.com/pages/publications/84893861222
U2 - 10.1016/j.ibiod.2014.01.012
DO - 10.1016/j.ibiod.2014.01.012
M3 - 文章
AN - SCOPUS:84893861222
SN - 0964-8305
VL - 89
SP - 67
EP - 73
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
ER -