Lock-in camera based heterodyne holography for ultrasound-modulated optical tomography inside dynamic scattering media

Yan Liu, Yuecheng Shen, Cheng Ma, Junhui Shi, Lihong V. Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Ultrasound-modulated optical tomography (UOT) images optical contrast deep inside scattering media. Heterodyne holography based UOT is a promising technique that uses a camera for parallel speckle detection. In previous works, the speed of data acquisition was limited by the low frame rates of conventional cameras. In addition, when the signal-to-background ratio was low, these cameras wasted most of their bits representing an informationless background, resulting in extremely low efficiencies in the use of bits. Here, using a lock-in camera, we increase the bit efficiency and reduce the data transfer load by digitizing only the signal after rejecting the background. Moreover, compared with the conventional four-frame based amplitude measurement method, our single-frame method is more immune to speckle decorrelation. Using lock-in camera based UOT with an integration time of 286 μs, we imaged an absorptive object buried inside a dynamic scattering medium exhibiting a speckle correlation time (τc) as short as 26 μs. Since our method can tolerate speckle decorrelation faster than that found in living biological tissue (τc ∼ 100-1000 μs), it is promising for in vivo deep tissue non-invasive imaging.

Original languageEnglish
Article number231106
JournalApplied Physics Letters
Volume108
Issue number23
DOIs
StatePublished - 6 Jun 2016
Externally publishedYes

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