lncRNA xist regulates osteoblast differentiation by sponging miR-19a-3p in aging-induced osteoporosis

Shijie Chen; Yuezhan Li; Shuang Zhi; Zhiyu Ding; Yan Huang; Weiguo Wang; Ruping Zheng; Haiyang Yu; Jianlong Wang; Minghua Hu; Jinglei Miao; Jinsong Li

doi:10.14336/AD.2019.0724

lncRNA xist regulates osteoblast differentiation by sponging miR-19a-3p in aging-induced osteoporosis

Shijie Chen, Yuezhan Li, Shuang Zhi, Zhiyu Ding, Yan Huang, Weiguo Wang, Ruping Zheng, Haiyang Yu, Jianlong Wang, Minghua Hu, Jinglei Miao, Jinsong Li

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

The switch between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) plays a key role in aging-induced osteoporosis. In this study, miR-19a-3p was obviously downregulated in BMSCs from aged humans and mice. Overexpressed miR-19a-3p evidently reduced aging-induced bone loss in mice and promoted osteogenic differentiation of BMSCs, while silenced miR-19a-3p manifestly increased aging-induced bone loss in mice and repressed osteogenic differentiation of BMSCs. Hoxa5 was significantly downregulated in the BMSCs from aged mice and contribute to miR-19a-3p-induced osteoblast differentiation as a direct target gene of miR-19a-3p. Furthermore, lncRNA Xist was found as a sponge of miR-19a-3p to repress BMSCs osteogenic differentiation. In conclusion, our study reveals the critical role of the lncRNA Xist/miR-19a-3p/Hoxa5 pathway in aging-induced osteogenic differentiation of BMSCs, indicating the potential therapeutic target for osteoporosis.

Original language	English
Pages (from-to)	1058-1068
Number of pages	11
Journal	Aging and Disease
Volume	11
Issue number	5
DOIs	https://doi.org/10.14336/AD.2019.0724
State	Published - Oct 2020

Keywords

BMSCs
Hoxa5
LncRNA Xist
MiR-19a-3p
Osteoporosis

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@article{e629ef48f8854904855e292de1b7c15c,

title = "lncRNA xist regulates osteoblast differentiation by sponging miR-19a-3p in aging-induced osteoporosis",

abstract = "The switch between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) plays a key role in aging-induced osteoporosis. In this study, miR-19a-3p was obviously downregulated in BMSCs from aged humans and mice. Overexpressed miR-19a-3p evidently reduced aging-induced bone loss in mice and promoted osteogenic differentiation of BMSCs, while silenced miR-19a-3p manifestly increased aging-induced bone loss in mice and repressed osteogenic differentiation of BMSCs. Hoxa5 was significantly downregulated in the BMSCs from aged mice and contribute to miR-19a-3p-induced osteoblast differentiation as a direct target gene of miR-19a-3p. Furthermore, lncRNA Xist was found as a sponge of miR-19a-3p to repress BMSCs osteogenic differentiation. In conclusion, our study reveals the critical role of the lncRNA Xist/miR-19a-3p/Hoxa5 pathway in aging-induced osteogenic differentiation of BMSCs, indicating the potential therapeutic target for osteoporosis.",

keywords = "BMSCs, Hoxa5, LncRNA Xist, MiR-19a-3p, Osteoporosis",

author = "Shijie Chen and Yuezhan Li and Shuang Zhi and Zhiyu Ding and Yan Huang and Weiguo Wang and Ruping Zheng and Haiyang Yu and Jianlong Wang and Minghua Hu and Jinglei Miao and Jinsong Li",

note = "Publisher Copyright: {\textcopyright} 2019 Chen S et al.",

year = "2020",

month = oct,

doi = "10.14336/AD.2019.0724",

language = "英语",

volume = "11",

pages = "1058--1068",

journal = "Aging and Disease",

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publisher = "International Society on Aging and Disease",

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TY - JOUR

T1 - lncRNA xist regulates osteoblast differentiation by sponging miR-19a-3p in aging-induced osteoporosis

AU - Chen, Shijie

AU - Li, Yuezhan

AU - Zhi, Shuang

AU - Ding, Zhiyu

AU - Huang, Yan

AU - Wang, Weiguo

AU - Zheng, Ruping

AU - Yu, Haiyang

AU - Wang, Jianlong

AU - Hu, Minghua

AU - Miao, Jinglei

AU - Li, Jinsong

PY - 2020/10

Y1 - 2020/10

N2 - The switch between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) plays a key role in aging-induced osteoporosis. In this study, miR-19a-3p was obviously downregulated in BMSCs from aged humans and mice. Overexpressed miR-19a-3p evidently reduced aging-induced bone loss in mice and promoted osteogenic differentiation of BMSCs, while silenced miR-19a-3p manifestly increased aging-induced bone loss in mice and repressed osteogenic differentiation of BMSCs. Hoxa5 was significantly downregulated in the BMSCs from aged mice and contribute to miR-19a-3p-induced osteoblast differentiation as a direct target gene of miR-19a-3p. Furthermore, lncRNA Xist was found as a sponge of miR-19a-3p to repress BMSCs osteogenic differentiation. In conclusion, our study reveals the critical role of the lncRNA Xist/miR-19a-3p/Hoxa5 pathway in aging-induced osteogenic differentiation of BMSCs, indicating the potential therapeutic target for osteoporosis.

AB - The switch between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) plays a key role in aging-induced osteoporosis. In this study, miR-19a-3p was obviously downregulated in BMSCs from aged humans and mice. Overexpressed miR-19a-3p evidently reduced aging-induced bone loss in mice and promoted osteogenic differentiation of BMSCs, while silenced miR-19a-3p manifestly increased aging-induced bone loss in mice and repressed osteogenic differentiation of BMSCs. Hoxa5 was significantly downregulated in the BMSCs from aged mice and contribute to miR-19a-3p-induced osteoblast differentiation as a direct target gene of miR-19a-3p. Furthermore, lncRNA Xist was found as a sponge of miR-19a-3p to repress BMSCs osteogenic differentiation. In conclusion, our study reveals the critical role of the lncRNA Xist/miR-19a-3p/Hoxa5 pathway in aging-induced osteogenic differentiation of BMSCs, indicating the potential therapeutic target for osteoporosis.

KW - BMSCs

KW - Hoxa5

KW - LncRNA Xist

KW - MiR-19a-3p

KW - Osteoporosis

UR - https://www.scopus.com/pages/publications/85093646598

U2 - 10.14336/AD.2019.0724

DO - 10.14336/AD.2019.0724

M3 - 文章

AN - SCOPUS:85093646598

SN - 2152-5250

VL - 11

SP - 1058

EP - 1068

JO - Aging and Disease

JF - Aging and Disease

IS - 5

ER -

lncRNA xist regulates osteoblast differentiation by sponging miR-19a-3p in aging-induced osteoporosis

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Keywords

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