TY - JOUR
T1 - Liquid chromatography with amperometric detection using functionalized multi-wall carbon nanotube modified electrode for the determination of monoamine neurotransmitters and their metabolites
AU - Zhang, Wen
AU - Xie, Yunfeng
AU - Ai, Shiyun
AU - Wan, Fangli
AU - Wang, Jian
AU - Jin, Litong
AU - Jin, Jiye
PY - 2003/7/5
Y1 - 2003/7/5
N2 - The fabrication and application of a novel electrochemical detection (ED) method with the functionalized multi-wall carbon nanotubes (MWNTs) chemically modified electrode (CME) for liquid chromatography (LC) were described. The electrochemical behaviors of dopamine (DA) and other monoamine neurotransmitters at the CME were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results indicated that the CME exhibited efficient electrocatalytic effects on the current responses of monoamine neurotransmitters and their metabolites with high sensitivity, high stability and long-life activity. In LC-ED, DA, norepinephrine (NE), 3-methoxy-4-hydroxyphenylglycol (MHPG), 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA) had good and stable current responses at the CME. The linear ranges of seven analytes were over four orders of magnitude and the detection limits were 2.5.10-10 mol/l for DA, 2.5×10-10 mol/l for NE, 5.0.10-10 mol/l for MHPG, 3.0.10-10 mol/l for DOPAC, 3.5.10-10 mol/l for 5-HT, 6.0.10-10 mol/l for 5-HIAA, 1.25.10-9 mol/l for HVA. The application of this method coupled with microdialysis sampling for the determination of monoamine neurotransmitters and their metabolites in Parkinsonian patients' cerebrospinal fluid was satisfactory.
AB - The fabrication and application of a novel electrochemical detection (ED) method with the functionalized multi-wall carbon nanotubes (MWNTs) chemically modified electrode (CME) for liquid chromatography (LC) were described. The electrochemical behaviors of dopamine (DA) and other monoamine neurotransmitters at the CME were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results indicated that the CME exhibited efficient electrocatalytic effects on the current responses of monoamine neurotransmitters and their metabolites with high sensitivity, high stability and long-life activity. In LC-ED, DA, norepinephrine (NE), 3-methoxy-4-hydroxyphenylglycol (MHPG), 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA) had good and stable current responses at the CME. The linear ranges of seven analytes were over four orders of magnitude and the detection limits were 2.5.10-10 mol/l for DA, 2.5×10-10 mol/l for NE, 5.0.10-10 mol/l for MHPG, 3.0.10-10 mol/l for DOPAC, 3.5.10-10 mol/l for 5-HT, 6.0.10-10 mol/l for 5-HIAA, 1.25.10-9 mol/l for HVA. The application of this method coupled with microdialysis sampling for the determination of monoamine neurotransmitters and their metabolites in Parkinsonian patients' cerebrospinal fluid was satisfactory.
KW - Monoamine neurotransmitters
KW - Multi-wall carbon nanotube
UR - https://www.scopus.com/pages/publications/0038359593
U2 - 10.1016/S1570-0232(03)00227-7
DO - 10.1016/S1570-0232(03)00227-7
M3 - 文章
C2 - 12798181
AN - SCOPUS:0038359593
SN - 1570-0232
VL - 791
SP - 217
EP - 225
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1-2
ER -