Liquid chromatography - Electrochemical detector for the determination of glucose in rat brain combined with in vivo microdialysis

Guoyue Shi; Jiaxing Lu; Fang Xu; Hongguo Zhou; Litong Jin; Jiye Jin

doi:10.1016/S0003-2670(00)00753-4

Liquid chromatography - Electrochemical detector for the determination of glucose in rat brain combined with in vivo microdialysis

Guoyue Shi
, Jiaxing Lu
, Fang Xu
, Hongguo Zhou
, Litong Jin^*
, Jiye Jin

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

East China Normal University
Gifu University

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

In this paper, microdialysis sampling was combined with liquid chromatography with electrochemical detection (LC-ECD) and glucose in rat brain was determined. A cobalt tetraaminophthalocyanine (CoTAPC)/glucose oxidase (GOD)/Nafion film-coated glassy carbon electrode was used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors, without any interference from electroactive species such as L-ascorbate, urate, proteins. The signal current was linearly related to the glucose concentration in the range of 1.0x10^-6-5.0x10^-3mol/l at +0.5V (versus Ag/AgCl) with a detection limit of 5.0x10^-7mol/l. To measure glucose in rat brain, microdialysis sampling was employed. The dialysate from the microdialysis system was injected into system. The result was 0.74±0.10mmol/l (n=4). Copyright (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	131-136
Number of pages	6
Journal	Analytica Chimica Acta
Volume	413
Issue number	1-2
DOIs	https://doi.org/10.1016/S0003-2670(00)00753-4
State	Published - 18 May 2000

Keywords

CoTAPC/GOD/Nafion biosensor
Glucose
LC-ECD
Microdialysis sampling

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10.1016/S0003-2670(00)00753-4

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title = "Liquid chromatography - Electrochemical detector for the determination of glucose in rat brain combined with in vivo microdialysis",

abstract = "In this paper, microdialysis sampling was combined with liquid chromatography with electrochemical detection (LC-ECD) and glucose in rat brain was determined. A cobalt tetraaminophthalocyanine (CoTAPC)/glucose oxidase (GOD)/Nafion film-coated glassy carbon electrode was used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors, without any interference from electroactive species such as L-ascorbate, urate, proteins. The signal current was linearly related to the glucose concentration in the range of 1.0x10-6-5.0x10-3mol/l at +0.5V (versus Ag/AgCl) with a detection limit of 5.0x10-7mol/l. To measure glucose in rat brain, microdialysis sampling was employed. The dialysate from the microdialysis system was injected into system. The result was 0.74±0.10mmol/l (n=4). Copyright (C) 2000 Elsevier Science B.V.",

keywords = "CoTAPC/GOD/Nafion biosensor, Glucose, LC-ECD, Microdialysis sampling",

author = "Guoyue Shi and Jiaxing Lu and Fang Xu and Hongguo Zhou and Litong Jin and Jiye Jin",

year = "2000",

month = may,

day = "18",

doi = "10.1016/S0003-2670(00)00753-4",

language = "英语",

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T1 - Liquid chromatography - Electrochemical detector for the determination of glucose in rat brain combined with in vivo microdialysis

AU - Shi, Guoyue

AU - Lu, Jiaxing

AU - Xu, Fang

AU - Zhou, Hongguo

AU - Jin, Litong

AU - Jin, Jiye

PY - 2000/5/18

Y1 - 2000/5/18

N2 - In this paper, microdialysis sampling was combined with liquid chromatography with electrochemical detection (LC-ECD) and glucose in rat brain was determined. A cobalt tetraaminophthalocyanine (CoTAPC)/glucose oxidase (GOD)/Nafion film-coated glassy carbon electrode was used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors, without any interference from electroactive species such as L-ascorbate, urate, proteins. The signal current was linearly related to the glucose concentration in the range of 1.0x10-6-5.0x10-3mol/l at +0.5V (versus Ag/AgCl) with a detection limit of 5.0x10-7mol/l. To measure glucose in rat brain, microdialysis sampling was employed. The dialysate from the microdialysis system was injected into system. The result was 0.74±0.10mmol/l (n=4). Copyright (C) 2000 Elsevier Science B.V.

AB - In this paper, microdialysis sampling was combined with liquid chromatography with electrochemical detection (LC-ECD) and glucose in rat brain was determined. A cobalt tetraaminophthalocyanine (CoTAPC)/glucose oxidase (GOD)/Nafion film-coated glassy carbon electrode was used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors, without any interference from electroactive species such as L-ascorbate, urate, proteins. The signal current was linearly related to the glucose concentration in the range of 1.0x10-6-5.0x10-3mol/l at +0.5V (versus Ag/AgCl) with a detection limit of 5.0x10-7mol/l. To measure glucose in rat brain, microdialysis sampling was employed. The dialysate from the microdialysis system was injected into system. The result was 0.74±0.10mmol/l (n=4). Copyright (C) 2000 Elsevier Science B.V.

KW - CoTAPC/GOD/Nafion biosensor

KW - Glucose

KW - LC-ECD

KW - Microdialysis sampling

UR - https://www.scopus.com/pages/publications/0034682230

U2 - 10.1016/S0003-2670(00)00753-4

DO - 10.1016/S0003-2670(00)00753-4

M3 - 文章

AN - SCOPUS:0034682230

SN - 0003-2670

VL - 413

SP - 131

EP - 136

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1-2

ER -

Liquid chromatography - Electrochemical detector for the determination of glucose in rat brain combined with in vivo microdialysis

Abstract

Keywords

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