Lighting Up Healing: Green Light-Driven Dual Gasotransmitters Release against MRSA Wound Infections

Methicillin-resistant Staphylococcus aureus (MRSA) represents a major clinical challenge as a predominant pathogen causing chronic and difficult-to-treat wound infections. Effective strategies that simultaneously combat bacterial infection and promote tissue regeneration are essential yet challenging for managing MRSA-related wound complications. Herein, we report the design of a novel integrated dual-gas release molecular platform, which enable the phototriggered simultaneous release of NO and CO within a single molecular entity, i.e., an acylhydrazine-caged rhodamine (AhCR565). Green light initiates the cascade release of NO and CO from AhCR565, with a synchronized fluorescent turn-on signal, serving as an intrinsic calibration mechanism for real-time tracking of the localization, kinetics of dual gas release. The photodriven simultaneous release of NO and CO from AhCR565 demonstrates a superior antimicrobial effect against MRSA, while exhibiting excellent biocompatibility. By harnessing the synergistic and complementary bioactivities of NO and CO, AhCR565 effectively eradicates bacterial infections and promotes wound healing in a marine MRSA-infected skin wound model. This work highlights a promising noninvasive strategy for multigas simultaneous release therapy, providing a potent approach to tackling resistant bacterial infections and addressing broader biomedical challenges.