Layer-by-Layer self-assembled acetylcholinesterase/PAMAM-Au on CNTs modified electrode for sensing pesticides

Yunhe Qu; Qian Sun; Fei Xiao; Guoyue Shi; Litong Jin

doi:10.1016/j.bioelechem.2009.08.001

Layer-by-Layer self-assembled acetylcholinesterase/PAMAM-Au on CNTs modified electrode for sensing pesticides

Yunhe Qu
, Qian Sun
, Fei Xiao
, Guoyue Shi^*
, Litong Jin

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

In this paper, an acetylcholinesterase (AChE)/dendrimers polyamidoamine (PAMAM)-Au/Carbon nanotubes (CNTs) multilayer modified electrode based on LbL self-assembled technique was employed in the detection of carbofuran in samples. The configuration of the nanostructure on the electrode provided a favorable environment to the immobilization of AChE. The modified films also improved the electrocatalytic characteristics and electron transfer speed between the films and the surface of electrode. The PAMAM-Au nanoparticles were characterized by SEM and UV-VIS methods. A set of experimental conditions were also optimized for the detection of the pesticides. A linear response over carbofuran concentration in the range of 4.8 × 10^{- 9} M to 0.9 × 10^{- 7} M was exhibited with a detection limit of 4.0 × 10^{- 9} M. The biosensor showed high sensitivity, good stability and reproducibility with promising application.

Original language	English
Pages (from-to)	139-144
Number of pages	6
Journal	Bioelectrochemistry
Volume	77
Issue number	2
DOIs	https://doi.org/10.1016/j.bioelechem.2009.08.001
State	Published - Feb 2010

Keywords

Carbofuran
Inhibition
PAMAM-Au
Sensor

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10.1016/j.bioelechem.2009.08.001

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@article{f0316090b76a4e908eda19dc5a394da4,

title = "Layer-by-Layer self-assembled acetylcholinesterase/PAMAM-Au on CNTs modified electrode for sensing pesticides",

abstract = "In this paper, an acetylcholinesterase (AChE)/dendrimers polyamidoamine (PAMAM)-Au/Carbon nanotubes (CNTs) multilayer modified electrode based on LbL self-assembled technique was employed in the detection of carbofuran in samples. The configuration of the nanostructure on the electrode provided a favorable environment to the immobilization of AChE. The modified films also improved the electrocatalytic characteristics and electron transfer speed between the films and the surface of electrode. The PAMAM-Au nanoparticles were characterized by SEM and UV-VIS methods. A set of experimental conditions were also optimized for the detection of the pesticides. A linear response over carbofuran concentration in the range of 4.8 × 10- 9 M to 0.9 × 10- 7 M was exhibited with a detection limit of 4.0 × 10- 9 M. The biosensor showed high sensitivity, good stability and reproducibility with promising application.",

keywords = "Carbofuran, Inhibition, PAMAM-Au, Sensor",

author = "Yunhe Qu and Qian Sun and Fei Xiao and Guoyue Shi and Litong Jin",

year = "2010",

month = feb,

doi = "10.1016/j.bioelechem.2009.08.001",

language = "英语",

volume = "77",

pages = "139--144",

journal = "Bioelectrochemistry",

issn = "1567-5394",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Layer-by-Layer self-assembled acetylcholinesterase/PAMAM-Au on CNTs modified electrode for sensing pesticides

AU - Qu, Yunhe

AU - Sun, Qian

AU - Xiao, Fei

AU - Shi, Guoyue

AU - Jin, Litong

PY - 2010/2

Y1 - 2010/2

N2 - In this paper, an acetylcholinesterase (AChE)/dendrimers polyamidoamine (PAMAM)-Au/Carbon nanotubes (CNTs) multilayer modified electrode based on LbL self-assembled technique was employed in the detection of carbofuran in samples. The configuration of the nanostructure on the electrode provided a favorable environment to the immobilization of AChE. The modified films also improved the electrocatalytic characteristics and electron transfer speed between the films and the surface of electrode. The PAMAM-Au nanoparticles were characterized by SEM and UV-VIS methods. A set of experimental conditions were also optimized for the detection of the pesticides. A linear response over carbofuran concentration in the range of 4.8 × 10- 9 M to 0.9 × 10- 7 M was exhibited with a detection limit of 4.0 × 10- 9 M. The biosensor showed high sensitivity, good stability and reproducibility with promising application.

AB - In this paper, an acetylcholinesterase (AChE)/dendrimers polyamidoamine (PAMAM)-Au/Carbon nanotubes (CNTs) multilayer modified electrode based on LbL self-assembled technique was employed in the detection of carbofuran in samples. The configuration of the nanostructure on the electrode provided a favorable environment to the immobilization of AChE. The modified films also improved the electrocatalytic characteristics and electron transfer speed between the films and the surface of electrode. The PAMAM-Au nanoparticles were characterized by SEM and UV-VIS methods. A set of experimental conditions were also optimized for the detection of the pesticides. A linear response over carbofuran concentration in the range of 4.8 × 10- 9 M to 0.9 × 10- 7 M was exhibited with a detection limit of 4.0 × 10- 9 M. The biosensor showed high sensitivity, good stability and reproducibility with promising application.

KW - Carbofuran

KW - Inhibition

KW - PAMAM-Au

KW - Sensor

UR - https://www.scopus.com/pages/publications/74449087364

U2 - 10.1016/j.bioelechem.2009.08.001

DO - 10.1016/j.bioelechem.2009.08.001

M3 - 文章

C2 - 19733130

AN - SCOPUS:74449087364

SN - 1567-5394

VL - 77

SP - 139

EP - 144

JO - Bioelectrochemistry

JF - Bioelectrochemistry

IS - 2

ER -

Layer-by-Layer self-assembled acetylcholinesterase/PAMAM-Au on CNTs modified electrode for sensing pesticides

Abstract

Keywords

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