Label-free immunoassay for chloramphenicol based on hollow gold nanospheres/chitosan composite

Ningdan Zhang; Fei Xiao; Jing Bai; Yanjun Lai; Jie Hou; Yuezhong Xian; Litong Jin

doi:10.1016/j.talanta.2011.07.108

Label-free immunoassay for chloramphenicol based on hollow gold nanospheres/chitosan composite

Ningdan Zhang
, Fei Xiao
, Jing Bai
, Yanjun Lai
, Jie Hou
, Yuezhong Xian^*
, Litong Jin

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

A novel label-free electrochemical immunosensor for rapid determination of chloramphenicol (CAP) was fabricated by entrapping monoclonal antibody to chloramphenicol (anti-CAP) in hollow gold nanospheres (HGNs)/chitosan composite modified on a glassy carbon electrode. The hollow gold nanospheres (HGNs) were prepared by using Co nanoparticles as sacrificial templates and characterized by transmission electron microscopy (TEM). The changes of the electrode behavior after each fabrication step were investigated by electrochemical impedance spectroscopy (EIS) technique. Under optimal conditions, the proposed immunosensor has a sensitive response to CAP in a linear range of 0.1-1000 ng mL^-1 with the detection limit of 0.06 ng mL^-1. Accurate detection of CAP in real meat samples was demonstrated by comparison with conventional HPLC method. The proposed method was proven to be a feasible quantitative method for CAP analysis with the properties of simple preparation, stability, high sensitivity and selectivity.

Original language	English
Pages (from-to)	100-105
Number of pages	6
Journal	Talanta
Volume	87
Issue number	1
DOIs	https://doi.org/10.1016/j.talanta.2011.07.108
State	Published - 15 Dec 2011

Keywords

Chitosan
Chloramphenicol
Hollow gold nanospheres
Immunosensor

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10.1016/j.talanta.2011.07.108

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@article{180666356b0446ceaa87aad30b50b003,

title = "Label-free immunoassay for chloramphenicol based on hollow gold nanospheres/chitosan composite",

abstract = "A novel label-free electrochemical immunosensor for rapid determination of chloramphenicol (CAP) was fabricated by entrapping monoclonal antibody to chloramphenicol (anti-CAP) in hollow gold nanospheres (HGNs)/chitosan composite modified on a glassy carbon electrode. The hollow gold nanospheres (HGNs) were prepared by using Co nanoparticles as sacrificial templates and characterized by transmission electron microscopy (TEM). The changes of the electrode behavior after each fabrication step were investigated by electrochemical impedance spectroscopy (EIS) technique. Under optimal conditions, the proposed immunosensor has a sensitive response to CAP in a linear range of 0.1-1000 ng mL-1 with the detection limit of 0.06 ng mL-1. Accurate detection of CAP in real meat samples was demonstrated by comparison with conventional HPLC method. The proposed method was proven to be a feasible quantitative method for CAP analysis with the properties of simple preparation, stability, high sensitivity and selectivity.",

keywords = "Chitosan, Chloramphenicol, Hollow gold nanospheres, Immunosensor",

author = "Ningdan Zhang and Fei Xiao and Jing Bai and Yanjun Lai and Jie Hou and Yuezhong Xian and Litong Jin",

year = "2011",

month = dec,

day = "15",

doi = "10.1016/j.talanta.2011.07.108",

language = "英语",

volume = "87",

pages = "100--105",

journal = "Talanta",

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TY - JOUR

T1 - Label-free immunoassay for chloramphenicol based on hollow gold nanospheres/chitosan composite

AU - Zhang, Ningdan

AU - Xiao, Fei

AU - Bai, Jing

AU - Lai, Yanjun

AU - Hou, Jie

AU - Xian, Yuezhong

AU - Jin, Litong

PY - 2011/12/15

Y1 - 2011/12/15

N2 - A novel label-free electrochemical immunosensor for rapid determination of chloramphenicol (CAP) was fabricated by entrapping monoclonal antibody to chloramphenicol (anti-CAP) in hollow gold nanospheres (HGNs)/chitosan composite modified on a glassy carbon electrode. The hollow gold nanospheres (HGNs) were prepared by using Co nanoparticles as sacrificial templates and characterized by transmission electron microscopy (TEM). The changes of the electrode behavior after each fabrication step were investigated by electrochemical impedance spectroscopy (EIS) technique. Under optimal conditions, the proposed immunosensor has a sensitive response to CAP in a linear range of 0.1-1000 ng mL-1 with the detection limit of 0.06 ng mL-1. Accurate detection of CAP in real meat samples was demonstrated by comparison with conventional HPLC method. The proposed method was proven to be a feasible quantitative method for CAP analysis with the properties of simple preparation, stability, high sensitivity and selectivity.

AB - A novel label-free electrochemical immunosensor for rapid determination of chloramphenicol (CAP) was fabricated by entrapping monoclonal antibody to chloramphenicol (anti-CAP) in hollow gold nanospheres (HGNs)/chitosan composite modified on a glassy carbon electrode. The hollow gold nanospheres (HGNs) were prepared by using Co nanoparticles as sacrificial templates and characterized by transmission electron microscopy (TEM). The changes of the electrode behavior after each fabrication step were investigated by electrochemical impedance spectroscopy (EIS) technique. Under optimal conditions, the proposed immunosensor has a sensitive response to CAP in a linear range of 0.1-1000 ng mL-1 with the detection limit of 0.06 ng mL-1. Accurate detection of CAP in real meat samples was demonstrated by comparison with conventional HPLC method. The proposed method was proven to be a feasible quantitative method for CAP analysis with the properties of simple preparation, stability, high sensitivity and selectivity.

KW - Chitosan

KW - Chloramphenicol

KW - Hollow gold nanospheres

KW - Immunosensor

UR - https://www.scopus.com/pages/publications/81355148454

U2 - 10.1016/j.talanta.2011.07.108

DO - 10.1016/j.talanta.2011.07.108

M3 - 文章

AN - SCOPUS:81355148454

SN - 0039-9140

VL - 87

SP - 100

EP - 105

JO - Talanta

JF - Talanta

IS - 1

ER -

Label-free immunoassay for chloramphenicol based on hollow gold nanospheres/chitosan composite

Abstract

Keywords

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