Label-free DNA sensor construction using self-assembled poly(o-methoxyaniline) hollow nanospheres

Jing Sui; Lijuan Zhang; Hui Peng

doi:10.1016/j.eurpolymj.2012.09.009

Label-free DNA sensor construction using self-assembled poly(o-methoxyaniline) hollow nanospheres

Jing Sui
, Lijuan Zhang^*
, Hui Peng

^*Corresponding author for this work

School of Physics and Electronic Science

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Poly(o-methoxyaniline) (POMA) hollow nanospheres diameter in 210 nm were prepared in a solution of poly(methyl vinyl etheralt-maleic acid) (PMVEA) by a self-assembly process using ammonium persulfate (APS) as the oxidant. The morphology and the amount of nanospheres of POMA in the final products are highly affected by the molar ratio of APS to o-methoxyaniline (OMA) and the concentration of OMA. The structure and property of POMA hollow nanospheres were characterized by FTIR and cyclic voltammetry. We constructed a label-free oligonucleotide (ODN) sensor using PMVEA-doped POMA nanospheres: PMVEA provides a high density of carboxylic acid groups for covalent grafting of the ODN probe. The hybridization of the complementary ODNs can be clearly detected by the decrease in the impedance without the need for an indicator or any sample modifications.

Original language	English
Pages (from-to)	139-146
Number of pages	8
Journal	European Polymer Journal
Volume	49
Issue number	1
DOIs	https://doi.org/10.1016/j.eurpolymj.2012.09.009
State	Published - Jan 2013

Keywords

Hollow nanospheres
Oligonucleotide sensor
Poly(o-methoxyaniline)

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10.1016/j.eurpolymj.2012.09.009

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title = "Label-free DNA sensor construction using self-assembled poly(o-methoxyaniline) hollow nanospheres",

abstract = "Poly(o-methoxyaniline) (POMA) hollow nanospheres diameter in 210 nm were prepared in a solution of poly(methyl vinyl etheralt-maleic acid) (PMVEA) by a self-assembly process using ammonium persulfate (APS) as the oxidant. The morphology and the amount of nanospheres of POMA in the final products are highly affected by the molar ratio of APS to o-methoxyaniline (OMA) and the concentration of OMA. The structure and property of POMA hollow nanospheres were characterized by FTIR and cyclic voltammetry. We constructed a label-free oligonucleotide (ODN) sensor using PMVEA-doped POMA nanospheres: PMVEA provides a high density of carboxylic acid groups for covalent grafting of the ODN probe. The hybridization of the complementary ODNs can be clearly detected by the decrease in the impedance without the need for an indicator or any sample modifications.",

keywords = "Hollow nanospheres, Oligonucleotide sensor, Poly(o-methoxyaniline)",

author = "Jing Sui and Lijuan Zhang and Hui Peng",

year = "2013",

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doi = "10.1016/j.eurpolymj.2012.09.009",

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volume = "49",

pages = "139--146",

journal = "European Polymer Journal",

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TY - JOUR

T1 - Label-free DNA sensor construction using self-assembled poly(o-methoxyaniline) hollow nanospheres

AU - Sui, Jing

AU - Zhang, Lijuan

AU - Peng, Hui

PY - 2013/1

Y1 - 2013/1

N2 - Poly(o-methoxyaniline) (POMA) hollow nanospheres diameter in 210 nm were prepared in a solution of poly(methyl vinyl etheralt-maleic acid) (PMVEA) by a self-assembly process using ammonium persulfate (APS) as the oxidant. The morphology and the amount of nanospheres of POMA in the final products are highly affected by the molar ratio of APS to o-methoxyaniline (OMA) and the concentration of OMA. The structure and property of POMA hollow nanospheres were characterized by FTIR and cyclic voltammetry. We constructed a label-free oligonucleotide (ODN) sensor using PMVEA-doped POMA nanospheres: PMVEA provides a high density of carboxylic acid groups for covalent grafting of the ODN probe. The hybridization of the complementary ODNs can be clearly detected by the decrease in the impedance without the need for an indicator or any sample modifications.

AB - Poly(o-methoxyaniline) (POMA) hollow nanospheres diameter in 210 nm were prepared in a solution of poly(methyl vinyl etheralt-maleic acid) (PMVEA) by a self-assembly process using ammonium persulfate (APS) as the oxidant. The morphology and the amount of nanospheres of POMA in the final products are highly affected by the molar ratio of APS to o-methoxyaniline (OMA) and the concentration of OMA. The structure and property of POMA hollow nanospheres were characterized by FTIR and cyclic voltammetry. We constructed a label-free oligonucleotide (ODN) sensor using PMVEA-doped POMA nanospheres: PMVEA provides a high density of carboxylic acid groups for covalent grafting of the ODN probe. The hybridization of the complementary ODNs can be clearly detected by the decrease in the impedance without the need for an indicator or any sample modifications.

KW - Hollow nanospheres

KW - Oligonucleotide sensor

KW - Poly(o-methoxyaniline)

UR - https://www.scopus.com/pages/publications/84872326100

U2 - 10.1016/j.eurpolymj.2012.09.009

DO - 10.1016/j.eurpolymj.2012.09.009

M3 - 文章

AN - SCOPUS:84872326100

SN - 0014-3057

VL - 49

SP - 139

EP - 146

JO - European Polymer Journal

JF - European Polymer Journal

IS - 1

ER -

Label-free DNA sensor construction using self-assembled poly(o-methoxyaniline) hollow nanospheres

Abstract

Keywords

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