Label-free detection of DNA hybridization based on a novel functionalized conducting polymer

Hui Peng; Christian Soeller; Nicholas A. Vigar; Vittorio Caprio; Jadranka Travas-Sejdic

doi:10.1016/j.bios.2006.07.010

Label-free detection of DNA hybridization based on a novel functionalized conducting polymer

Hui Peng^*
, Christian Soeller
, Nicholas A. Vigar
, Vittorio Caprio
, Jadranka Travas-Sejdic

^*Corresponding author for this work

The University of Auckland

Research output: Contribution to journal › Article › peer-review

104 Scopus citations

Abstract

A new functionalized pyrrole monomer, 3-pyrrolylacrylic acid (PAA) was synthesized. It was used to prepare a copolymer with pyrrole, poly(Py-co-PAA), which was investigated by reflective FT-IR, UV-vis spectroscopy and cyclic voltammetry. A label-free DNA sensor was prepared based on a poly(Py-co-PAA) film. Hybridization with complementary and non-complementary DNA targets was studied by electrochemical impedance spectroscopy. Results show a significant increase in the charge-transfer resistance upon addition of complementary target. The impedance spectra were analyzed by using a modified Randles and Ershler equivalent circuit model. The change in charge-transfer resistance that was used as an index of sensor response was found to be linear with logarithmic target concentration in the range of 2 × 10^-9 to 2 × 10^-7 M. The detection limit was 0.98 nM.

Original language	English
Pages (from-to)	1868-1873
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	22
Issue number	9-10
DOIs	https://doi.org/10.1016/j.bios.2006.07.010
State	Published - 15 Apr 2007
Externally published	Yes

Keywords

Electrochemical impedance spectroscopy
Functionalized polypyrrole
Hybridization
Label-free DNA detection

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10.1016/j.bios.2006.07.010

Cite this

@article{16752cf16a95444b8a3cd4268f9ff403,

title = "Label-free detection of DNA hybridization based on a novel functionalized conducting polymer",

abstract = "A new functionalized pyrrole monomer, 3-pyrrolylacrylic acid (PAA) was synthesized. It was used to prepare a copolymer with pyrrole, poly(Py-co-PAA), which was investigated by reflective FT-IR, UV-vis spectroscopy and cyclic voltammetry. A label-free DNA sensor was prepared based on a poly(Py-co-PAA) film. Hybridization with complementary and non-complementary DNA targets was studied by electrochemical impedance spectroscopy. Results show a significant increase in the charge-transfer resistance upon addition of complementary target. The impedance spectra were analyzed by using a modified Randles and Ershler equivalent circuit model. The change in charge-transfer resistance that was used as an index of sensor response was found to be linear with logarithmic target concentration in the range of 2 × 10-9 to 2 × 10-7 M. The detection limit was 0.98 nM.",

keywords = "Electrochemical impedance spectroscopy, Functionalized polypyrrole, Hybridization, Label-free DNA detection",

author = "Hui Peng and Christian Soeller and Vigar, \{Nicholas A.\} and Vittorio Caprio and Jadranka Travas-Sejdic",

year = "2007",

month = apr,

day = "15",

doi = "10.1016/j.bios.2006.07.010",

language = "英语",

volume = "22",

pages = "1868--1873",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Ltd",

number = "9-10",

}

TY - JOUR

T1 - Label-free detection of DNA hybridization based on a novel functionalized conducting polymer

AU - Peng, Hui

AU - Soeller, Christian

AU - Vigar, Nicholas A.

AU - Caprio, Vittorio

AU - Travas-Sejdic, Jadranka

PY - 2007/4/15

Y1 - 2007/4/15

N2 - A new functionalized pyrrole monomer, 3-pyrrolylacrylic acid (PAA) was synthesized. It was used to prepare a copolymer with pyrrole, poly(Py-co-PAA), which was investigated by reflective FT-IR, UV-vis spectroscopy and cyclic voltammetry. A label-free DNA sensor was prepared based on a poly(Py-co-PAA) film. Hybridization with complementary and non-complementary DNA targets was studied by electrochemical impedance spectroscopy. Results show a significant increase in the charge-transfer resistance upon addition of complementary target. The impedance spectra were analyzed by using a modified Randles and Ershler equivalent circuit model. The change in charge-transfer resistance that was used as an index of sensor response was found to be linear with logarithmic target concentration in the range of 2 × 10-9 to 2 × 10-7 M. The detection limit was 0.98 nM.

AB - A new functionalized pyrrole monomer, 3-pyrrolylacrylic acid (PAA) was synthesized. It was used to prepare a copolymer with pyrrole, poly(Py-co-PAA), which was investigated by reflective FT-IR, UV-vis spectroscopy and cyclic voltammetry. A label-free DNA sensor was prepared based on a poly(Py-co-PAA) film. Hybridization with complementary and non-complementary DNA targets was studied by electrochemical impedance spectroscopy. Results show a significant increase in the charge-transfer resistance upon addition of complementary target. The impedance spectra were analyzed by using a modified Randles and Ershler equivalent circuit model. The change in charge-transfer resistance that was used as an index of sensor response was found to be linear with logarithmic target concentration in the range of 2 × 10-9 to 2 × 10-7 M. The detection limit was 0.98 nM.

KW - Electrochemical impedance spectroscopy

KW - Functionalized polypyrrole

KW - Hybridization

KW - Label-free DNA detection

UR - https://www.scopus.com/pages/publications/33847417895

U2 - 10.1016/j.bios.2006.07.010

DO - 10.1016/j.bios.2006.07.010

M3 - 文章

C2 - 16919441

AN - SCOPUS:33847417895

SN - 0956-5663

VL - 22

SP - 1868

EP - 1873

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 9-10

ER -

Label-free detection of DNA hybridization based on a novel functionalized conducting polymer

Abstract

Keywords

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