Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors

A. Gao; N. Lu; P. Dai; T. Li; Y. Wang

doi:10.1109/Transducers.2013.6627299

Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors

A. Gao
, N. Lu
, P. Dai
, T. Li
, Y. Wang

CAS - Shanghai Institute of Microsystem and Information Technology
University of Chinese Academy of Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

7 Scopus citations

Abstract

MicroRNAs (miRNAs) have been regarded as biomarkers for early-stage diagnosis of cancer. Due to their short length, low abundance, and very similar sequences, an attractive approach that is ultrasensitive and label-free is extremely demanded. Here we report the design of a novel semiconducting silicon nanowire field-effect transistor (SiNW-FET) biosensor array for ultrasensitive label-free and real-time detection of miR-21. Optical lithography and anisotropic self-stop etching were employed to guarantee low cost batch production for SiNWs. The working conditions of the nanosensor were firstly optimized through pH sensing experiment. This SiNW-FET nanosensor under optimal conditions revealed ultrahigh sensitivity for repaid and reliable detection of 1 fM miR-21 and high specificity for single-nucleotide polymorphism discrimination.

Original language	English
Title of host publication	2013 Transducers and Eurosensors XXVII
Subtitle of host publication	The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages	2439-2442
Number of pages	4
DOIs	https://doi.org/10.1109/Transducers.2013.6627299
State	Published - 2013
Externally published	Yes
Event	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain Duration: 16 Jun 2013 → 20 Jun 2013

Publication series

Name	2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Conference

Conference	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/Territory	Spain
City	Barcelona
Period	16/06/13 → 20/06/13

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

SiNWs-FET
label-free
miRNA
ultrasensitive

Access to Document

10.1109/Transducers.2013.6627299

Cite this

Gao, A., Lu, N., Dai, P., Li, T., & Wang, Y. (2013). Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 2439-2442). Article 6627299 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013). https://doi.org/10.1109/Transducers.2013.6627299

Gao, A. ; Lu, N. ; Dai, P. et al. / Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors. 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. pp. 2439-2442 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013).

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title = "Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors",

abstract = "MicroRNAs (miRNAs) have been regarded as biomarkers for early-stage diagnosis of cancer. Due to their short length, low abundance, and very similar sequences, an attractive approach that is ultrasensitive and label-free is extremely demanded. Here we report the design of a novel semiconducting silicon nanowire field-effect transistor (SiNW-FET) biosensor array for ultrasensitive label-free and real-time detection of miR-21. Optical lithography and anisotropic self-stop etching were employed to guarantee low cost batch production for SiNWs. The working conditions of the nanosensor were firstly optimized through pH sensing experiment. This SiNW-FET nanosensor under optimal conditions revealed ultrahigh sensitivity for repaid and reliable detection of 1 fM miR-21 and high specificity for single-nucleotide polymorphism discrimination.",

keywords = "SiNWs-FET, label-free, miRNA, ultrasensitive",

author = "A. Gao and N. Lu and P. Dai and T. Li and Y. Wang",

year = "2013",

doi = "10.1109/Transducers.2013.6627299",

language = "英语",

isbn = "9781467359818",

series = "2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013",

pages = "2439--2442",

booktitle = "2013 Transducers and Eurosensors XXVII",

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}

Gao, A, Lu, N, Dai, P, Li, T & Wang, Y 2013, Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6627299, 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, pp. 2439-2442, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, Spain, 16/06/13. https://doi.org/10.1109/Transducers.2013.6627299

Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors. / Gao, A.; Lu, N.; Dai, P. et al.
2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 2439-2442 6627299 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors

AU - Gao, A.

AU - Lu, N.

AU - Dai, P.

AU - Li, T.

AU - Wang, Y.

PY - 2013

Y1 - 2013

N2 - MicroRNAs (miRNAs) have been regarded as biomarkers for early-stage diagnosis of cancer. Due to their short length, low abundance, and very similar sequences, an attractive approach that is ultrasensitive and label-free is extremely demanded. Here we report the design of a novel semiconducting silicon nanowire field-effect transistor (SiNW-FET) biosensor array for ultrasensitive label-free and real-time detection of miR-21. Optical lithography and anisotropic self-stop etching were employed to guarantee low cost batch production for SiNWs. The working conditions of the nanosensor were firstly optimized through pH sensing experiment. This SiNW-FET nanosensor under optimal conditions revealed ultrahigh sensitivity for repaid and reliable detection of 1 fM miR-21 and high specificity for single-nucleotide polymorphism discrimination.

AB - MicroRNAs (miRNAs) have been regarded as biomarkers for early-stage diagnosis of cancer. Due to their short length, low abundance, and very similar sequences, an attractive approach that is ultrasensitive and label-free is extremely demanded. Here we report the design of a novel semiconducting silicon nanowire field-effect transistor (SiNW-FET) biosensor array for ultrasensitive label-free and real-time detection of miR-21. Optical lithography and anisotropic self-stop etching were employed to guarantee low cost batch production for SiNWs. The working conditions of the nanosensor were firstly optimized through pH sensing experiment. This SiNW-FET nanosensor under optimal conditions revealed ultrahigh sensitivity for repaid and reliable detection of 1 fM miR-21 and high specificity for single-nucleotide polymorphism discrimination.

KW - SiNWs-FET

KW - label-free

KW - miRNA

KW - ultrasensitive

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DO - 10.1109/Transducers.2013.6627299

M3 - 会议稿件

AN - SCOPUS:84891683198

SN - 9781467359818

T3 - 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

SP - 2439

EP - 2442

BT - 2013 Transducers and Eurosensors XXVII

T2 - 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Y2 - 16 June 2013 through 20 June 2013

ER -

Gao A, Lu N, Dai P, Li T, Wang Y. Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 2439-2442. 6627299. (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013). doi: 10.1109/Transducers.2013.6627299

Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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