TY - JOUR
T1 - IXL, a new subunit of the mammalian Mediator complex, functions as a transcriptional suppressor
AU - Wang, Yuequn
AU - Li, Yongqing
AU - Zeng, Weiqi
AU - Zhu, Chuanbing
AU - Xiao, Jing
AU - Yuan, Wuzhou
AU - Wang, Ying
AU - Cai, Zhenyu
AU - Zhou, Junmei
AU - Liu, Mingyao
AU - Wu, Xiushan
PY - 2004/12/24
Y1 - 2004/12/24
N2 - Transcription factors play an essential role in altering gene expression. Much progress about transcription factors has been made toward the understanding of normal physiological processes, embryonic development, and human diseases. In the present study, we report the identification and characterization of a novel human gene, (Drosophila) intersex-like (IXL), from a human embryonic heart cDNA library. IXL encodes a putative protein of 221 amino acids. The protein is conserved across different species during evolution. Northern blot analysis indicates that IXL is expressed in various tissues of human adult and during three different developmental stages of embryo. In COS-7 cells, IXL protein is localized to nucleus and cytoplasm. IXL is a transcription suppressor when fused to Gal-4 DNA-binding domain and cotransfected with VP-16. Overexpression of IXL in COS-7 cells inhibits the transcriptional activities of SRE and AP-1, suggesting that the IXL protein may act as a transcriptional suppressor in mitogen-activated protein kinase signaling pathway to mediate cellular functions.
AB - Transcription factors play an essential role in altering gene expression. Much progress about transcription factors has been made toward the understanding of normal physiological processes, embryonic development, and human diseases. In the present study, we report the identification and characterization of a novel human gene, (Drosophila) intersex-like (IXL), from a human embryonic heart cDNA library. IXL encodes a putative protein of 221 amino acids. The protein is conserved across different species during evolution. Northern blot analysis indicates that IXL is expressed in various tissues of human adult and during three different developmental stages of embryo. In COS-7 cells, IXL protein is localized to nucleus and cytoplasm. IXL is a transcription suppressor when fused to Gal-4 DNA-binding domain and cotransfected with VP-16. Overexpression of IXL in COS-7 cells inhibits the transcriptional activities of SRE and AP-1, suggesting that the IXL protein may act as a transcriptional suppressor in mitogen-activated protein kinase signaling pathway to mediate cellular functions.
KW - AP-1
KW - IXL
KW - MAPK signaling pathway
KW - SRE
KW - Transcriptional suppressor
UR - https://www.scopus.com/pages/publications/8844250940
U2 - 10.1016/j.bbrc.2004.10.159
DO - 10.1016/j.bbrc.2004.10.159
M3 - 文章
C2 - 15555573
AN - SCOPUS:8844250940
SN - 0006-291X
VL - 325
SP - 1330
EP - 1338
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -
