Isolation and characterization of polymorphic microsatellite markers in Metasequoia glyptostroboides (Taxodiaceae)

Min Yan Cui; Shuo Yu; Min Liu; Yuan Yuan Li

doi:10.1007/s12686-009-9132-6

Isolation and characterization of polymorphic microsatellite markers in Metasequoia glyptostroboides (Taxodiaceae)

Min Yan Cui, Shuo Yu, Min Liu, Yuan Yuan Li^*

^*Corresponding author for this work

School of Ecology and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Metasequoia glyptostroboides is an endangered plant endemic to China. Eleven polymorphic microsatellite loci were successfully isolated from M. glyptostroboides. The number of alleles per locus ranged from 2 to 6. The observed (HO) and expected (HE) heterozygosities ranged from 0.1842 to 0.8421 and 0.2389 to 0.6639, respectively. Polymorphism information content ranged from 0.2172 to 0.5901. No significant linkage disequilibrium was observed between the loci. Four loci showed significant deviations from Hardy-Weinberg equilibrium. These 11 effective primer pairs are expected to be useful for further studies on population genetics, mating system analysis, and conservation genetics in M. glyptostroboides.

Original language	English
Pages (from-to)	19-21
Number of pages	3
Journal	Conservation Genetics Resources
Volume	2
Issue number	SUPPL.1
DOIs	https://doi.org/10.1007/s12686-009-9132-6
State	Published - Sep 2010

Keywords

Conservation
Genetic diversity
Metasequoia glyptostroboides
Microsatellite loci

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10.1007/s12686-009-9132-6

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title = "Isolation and characterization of polymorphic microsatellite markers in Metasequoia glyptostroboides (Taxodiaceae)",

abstract = "Metasequoia glyptostroboides is an endangered plant endemic to China. Eleven polymorphic microsatellite loci were successfully isolated from M. glyptostroboides. The number of alleles per locus ranged from 2 to 6. The observed (HO) and expected (HE) heterozygosities ranged from 0.1842 to 0.8421 and 0.2389 to 0.6639, respectively. Polymorphism information content ranged from 0.2172 to 0.5901. No significant linkage disequilibrium was observed between the loci. Four loci showed significant deviations from Hardy-Weinberg equilibrium. These 11 effective primer pairs are expected to be useful for further studies on population genetics, mating system analysis, and conservation genetics in M. glyptostroboides.",

keywords = "Conservation, Genetic diversity, Metasequoia glyptostroboides, Microsatellite loci",

author = "Cui, \{Min Yan\} and Shuo Yu and Min Liu and Li, \{Yuan Yuan\}",

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doi = "10.1007/s12686-009-9132-6",

language = "英语",

volume = "2",

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TY - JOUR

T1 - Isolation and characterization of polymorphic microsatellite markers in Metasequoia glyptostroboides (Taxodiaceae)

AU - Cui, Min Yan

AU - Yu, Shuo

AU - Liu, Min

AU - Li, Yuan Yuan

PY - 2010/9

Y1 - 2010/9

N2 - Metasequoia glyptostroboides is an endangered plant endemic to China. Eleven polymorphic microsatellite loci were successfully isolated from M. glyptostroboides. The number of alleles per locus ranged from 2 to 6. The observed (HO) and expected (HE) heterozygosities ranged from 0.1842 to 0.8421 and 0.2389 to 0.6639, respectively. Polymorphism information content ranged from 0.2172 to 0.5901. No significant linkage disequilibrium was observed between the loci. Four loci showed significant deviations from Hardy-Weinberg equilibrium. These 11 effective primer pairs are expected to be useful for further studies on population genetics, mating system analysis, and conservation genetics in M. glyptostroboides.

AB - Metasequoia glyptostroboides is an endangered plant endemic to China. Eleven polymorphic microsatellite loci were successfully isolated from M. glyptostroboides. The number of alleles per locus ranged from 2 to 6. The observed (HO) and expected (HE) heterozygosities ranged from 0.1842 to 0.8421 and 0.2389 to 0.6639, respectively. Polymorphism information content ranged from 0.2172 to 0.5901. No significant linkage disequilibrium was observed between the loci. Four loci showed significant deviations from Hardy-Weinberg equilibrium. These 11 effective primer pairs are expected to be useful for further studies on population genetics, mating system analysis, and conservation genetics in M. glyptostroboides.

KW - Conservation

KW - Genetic diversity

KW - Metasequoia glyptostroboides

KW - Microsatellite loci

UR - https://www.scopus.com/pages/publications/84859997170

U2 - 10.1007/s12686-009-9132-6

DO - 10.1007/s12686-009-9132-6

M3 - 文章

AN - SCOPUS:84859997170

SN - 1877-7252

VL - 2

SP - 19

EP - 21

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - SUPPL.1

ER -

Isolation and characterization of polymorphic microsatellite markers in Metasequoia glyptostroboides (Taxodiaceae)

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Keywords

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