Isolation and characterization of microsatellite markers for the endangered Monimopetalum chinense (Celastraceae)

Hai Sheng Li; Guo Wen Xie; Xiao Yong Chen

doi:10.1007/s10592-008-9767-y

Isolation and characterization of microsatellite markers for the endangered Monimopetalum chinense (Celastraceae)

Hai Sheng Li
, Guo Wen Xie
, Xiao Yong Chen^*

^*Corresponding author for this work

School of Ecology and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We describe the isolation and characterization of 13 polymorphic microsatellite loci in the endangered Monimopetalum chinense (Celastraceae) using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol in this paper. The 13 loci were tested in 20 individuals from two populations. The number of alleles per locus varied from 2 to 8, with an average of 3.8. The expected and observed heterozygosities ranged from 0.05 to 0.87 and from 0.05 to 0.55, respectively. Two of the 13 loci were biased from Hardy-Weinberg equilibrium. No significant linkage disequilibrium was found among all loci. These polymorphic SSR markers will be useful in studying population genetics of M. chinense.

Original language	English
Pages (from-to)	1497-1499
Number of pages	3
Journal	Conservation Genetics
Volume	10
Issue number	5
DOIs	https://doi.org/10.1007/s10592-008-9767-y
State	Published - Sep 2009

Keywords

Heterozygosity
Microsatellites
Monimopetalum chinense
Population genetics

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title = "Isolation and characterization of microsatellite markers for the endangered Monimopetalum chinense (Celastraceae)",

abstract = "We describe the isolation and characterization of 13 polymorphic microsatellite loci in the endangered Monimopetalum chinense (Celastraceae) using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol in this paper. The 13 loci were tested in 20 individuals from two populations. The number of alleles per locus varied from 2 to 8, with an average of 3.8. The expected and observed heterozygosities ranged from 0.05 to 0.87 and from 0.05 to 0.55, respectively. Two of the 13 loci were biased from Hardy-Weinberg equilibrium. No significant linkage disequilibrium was found among all loci. These polymorphic SSR markers will be useful in studying population genetics of M. chinense.",

keywords = "Heterozygosity, Microsatellites, Monimopetalum chinense, Population genetics",

author = "Li, \{Hai Sheng\} and Xie, \{Guo Wen\} and Chen, \{Xiao Yong\}",

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doi = "10.1007/s10592-008-9767-y",

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T1 - Isolation and characterization of microsatellite markers for the endangered Monimopetalum chinense (Celastraceae)

AU - Li, Hai Sheng

AU - Xie, Guo Wen

AU - Chen, Xiao Yong

PY - 2009/9

Y1 - 2009/9

N2 - We describe the isolation and characterization of 13 polymorphic microsatellite loci in the endangered Monimopetalum chinense (Celastraceae) using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol in this paper. The 13 loci were tested in 20 individuals from two populations. The number of alleles per locus varied from 2 to 8, with an average of 3.8. The expected and observed heterozygosities ranged from 0.05 to 0.87 and from 0.05 to 0.55, respectively. Two of the 13 loci were biased from Hardy-Weinberg equilibrium. No significant linkage disequilibrium was found among all loci. These polymorphic SSR markers will be useful in studying population genetics of M. chinense.

AB - We describe the isolation and characterization of 13 polymorphic microsatellite loci in the endangered Monimopetalum chinense (Celastraceae) using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol in this paper. The 13 loci were tested in 20 individuals from two populations. The number of alleles per locus varied from 2 to 8, with an average of 3.8. The expected and observed heterozygosities ranged from 0.05 to 0.87 and from 0.05 to 0.55, respectively. Two of the 13 loci were biased from Hardy-Weinberg equilibrium. No significant linkage disequilibrium was found among all loci. These polymorphic SSR markers will be useful in studying population genetics of M. chinense.

KW - Heterozygosity

KW - Microsatellites

KW - Monimopetalum chinense

KW - Population genetics

UR - https://www.scopus.com/pages/publications/70350532856

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DO - 10.1007/s10592-008-9767-y

M3 - 文章

AN - SCOPUS:70350532856

SN - 1566-0621

VL - 10

SP - 1497

EP - 1499

JO - Conservation Genetics

JF - Conservation Genetics

IS - 5

ER -

Isolation and characterization of microsatellite markers for the endangered Monimopetalum chinense (Celastraceae)

Abstract

Keywords

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