Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae)

Z. Z. Xiao; W. W. Chen; W. Bao; R. Wang; Y. Y. Li

doi:10.4238/gmr.15028322

Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae)

Z. Z. Xiao, W. W. Chen, W. Bao, R. Wang, Y. Y. Li

School of Ecology and Environmental Sciences

East China Normal University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populations. The number of alleles per locus ranged from 3 to 23. The observed and expected heterozygosities per locus were 0.033-0.773 and 0.138-0.924, respectively. These microsatellite loci will facilitate the studies on genetic variation, mating system, and gene flow of Q. fabri.

Original language	English
Article number	gmr.15028322
Journal	Genetics and Molecular Research
Volume	15
Issue number	2
DOIs	https://doi.org/10.4238/gmr.15028322
State	Published - 21 Jun 2016

Keywords

Ecological restoration
Fagaceae
Genetic diversity
Quercus fabri
Simple sequence repeat

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abstract = "Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populations. The number of alleles per locus ranged from 3 to 23. The observed and expected heterozygosities per locus were 0.033-0.773 and 0.138-0.924, respectively. These microsatellite loci will facilitate the studies on genetic variation, mating system, and gene flow of Q. fabri.",

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AU - Xiao, Z. Z.

AU - Chen, W. W.

AU - Bao, W.

AU - Wang, R.

AU - Li, Y. Y.

N1 - Publisher Copyright: © FUNPEC-RP.

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N2 - Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populations. The number of alleles per locus ranged from 3 to 23. The observed and expected heterozygosities per locus were 0.033-0.773 and 0.138-0.924, respectively. These microsatellite loci will facilitate the studies on genetic variation, mating system, and gene flow of Q. fabri.

AB - Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populations. The number of alleles per locus ranged from 3 to 23. The observed and expected heterozygosities per locus were 0.033-0.773 and 0.138-0.924, respectively. These microsatellite loci will facilitate the studies on genetic variation, mating system, and gene flow of Q. fabri.

KW - Ecological restoration

KW - Fagaceae

KW - Genetic diversity

KW - Quercus fabri

KW - Simple sequence repeat

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Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae)

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