Internal Calibration Potentiometric Aptasensors for Simultaneous Detection of Hg2+, Cd2+, and As3+ Based on a Screen-Printed Carbon Electrodes Array

  • Wanxin Tang
  • , Zhenzhen Wang
  • , Juan Yu
  • , Fan Zhang*
  • , Pingang He
  • *Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

An all-solid-state potentiometric aptasensor array based on a multichannel disposable screen-printed carbon electrode (SPCE) was demonstrated for the simultaneous detection of Hg2+, Cd2+, and As3+ by open circuit potential (OCP) technology. The potential of the channel with an internal calibration DNA sequence (IC-DNA) was employed as the internal calibration potential (ICP) to subtract the background signal generated by the detection system, providing a built-in correction methodology. As a result, the developed aptasensor array showed high sensitivity and accuracy for detecting Hg2+, Cd2+, and As3+ without mutual interference or interference from other ions. The linear response ranged from 2.5 pM to 2.5 μM, and the detection limits for Hg2+, Cd2+, and As3+ were 2.0, 0.62, and 0.17 pM, respectively. Furthermore, the potentiometric aptasensor array was successfully applied for the simultaneous detection of three ions in real samples. The results obtained from the developed approach agreed well with the results obtained from inductively coupled plasma mass spectrometry.

Original languageEnglish
Pages (from-to)8337-8344
Number of pages8
JournalAnalytical Chemistry
Volume90
Issue number14
DOIs
StatePublished - 17 Jul 2018

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