Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity

Min Zhang; Bin Cheng Yin; Xian Fei Wang; Bang Ce Ye

doi:10.1039/c0cc04887a

Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity

Min Zhang, Bin Cheng Yin, Xian Fei Wang, Bang Ce Ye^*

^*Corresponding author for this work

East China University of Science and Technology

Research output: Contribution to journal › Article › peer-review

218 Scopus citations

Abstract

We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.

Original language	English
Pages (from-to)	2399-2401
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	8
DOIs	https://doi.org/10.1039/c0cc04887a
State	Published - 7 Feb 2011
Externally published	Yes

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title = "Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity",

abstract = "We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.",

author = "Min Zhang and Yin, \{Bin Cheng\} and Wang, \{Xian Fei\} and Ye, \{Bang Ce\}",

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AU - Zhang, Min

AU - Yin, Bin Cheng

AU - Wang, Xian Fei

AU - Ye, Bang Ce

PY - 2011/2/7

Y1 - 2011/2/7

N2 - We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.

AB - We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.

UR - https://www.scopus.com/pages/publications/79751518967

U2 - 10.1039/c0cc04887a

DO - 10.1039/c0cc04887a

M3 - 文章

C2 - 21305066

AN - SCOPUS:79751518967

SN - 1359-7345

VL - 47

SP - 2399

EP - 2401

JO - Chemical Communications

JF - Chemical Communications

IS - 8

ER -

Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity

Abstract

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