Inhibitory effect of melittin on Na+, K+-ATPase from guinea pig myocardial mitochondria

S. Yang; X. M. Zhang; M. H. Jiang

Inhibitory effect of melittin on Na⁺, K⁺-ATPase from guinea pig myocardial mitochondria

S. Yang^*, X. M. Zhang, M. H. Jiang

^*Corresponding author for this work

Fudan University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

AIM: To investigate the effect of melittin (Mel) of Na⁺, K⁺-ATPase activity and it's kinetic mode of action on guinea pig myocardial mitochondria. METHODS: Effect of Mel on heart mitochondrial Na⁺, K⁺-ATPase activity was determined with colorimetry method. RESULTS: Mel inhibited Na⁺, K⁺-ATPase in a concentration and time dependent manner, IC₅₀ was 2.60 μmol/L. Kinetic studies of interaction between Mel and K⁺, Na⁺, ATP revealed that inhibitory effect of Mel was competitive with K⁺, but not with Na⁺ and ATP. CONCLUSION: Mel polypeptide potently inhibits Na⁺, K⁺-ATPase, possibly by binding to the K⁺ site.

Original language	English
Pages (from-to)	279-282
Number of pages	4
Journal	Acta Pharmacologica Sinica
Volume	22
Issue number	3
State	Published - 2001
Externally published	Yes

Keywords

Bee venoms
Melittin
Mitochondria
Myocardium
Na, K-exchanging ATPase

Cite this

@article{57c726fadb3d43859a7490137ed982b9,

title = "Inhibitory effect of melittin on Na+, K+-ATPase from guinea pig myocardial mitochondria",

abstract = "AIM: To investigate the effect of melittin (Mel) of Na+, K+-ATPase activity and it's kinetic mode of action on guinea pig myocardial mitochondria. METHODS: Effect of Mel on heart mitochondrial Na+, K+-ATPase activity was determined with colorimetry method. RESULTS: Mel inhibited Na+, K+-ATPase in a concentration and time dependent manner, IC50 was 2.60 μmol/L. Kinetic studies of interaction between Mel and K+, Na+, ATP revealed that inhibitory effect of Mel was competitive with K+, but not with Na+ and ATP. CONCLUSION: Mel polypeptide potently inhibits Na+, K+-ATPase, possibly by binding to the K+ site.",

keywords = "Bee venoms, Melittin, Mitochondria, Myocardium, Na, K-exchanging ATPase",

author = "S. Yang and Zhang, \{X. M.\} and Jiang, \{M. H.\}",

year = "2001",

language = "英语",

volume = "22",

pages = "279--282",

journal = "Acta Pharmacologica Sinica",

issn = "0253-9756",

publisher = "Springer Nature",

number = "3",

}

TY - JOUR

T1 - Inhibitory effect of melittin on Na+, K+-ATPase from guinea pig myocardial mitochondria

AU - Yang, S.

AU - Zhang, X. M.

AU - Jiang, M. H.

PY - 2001

Y1 - 2001

N2 - AIM: To investigate the effect of melittin (Mel) of Na+, K+-ATPase activity and it's kinetic mode of action on guinea pig myocardial mitochondria. METHODS: Effect of Mel on heart mitochondrial Na+, K+-ATPase activity was determined with colorimetry method. RESULTS: Mel inhibited Na+, K+-ATPase in a concentration and time dependent manner, IC50 was 2.60 μmol/L. Kinetic studies of interaction between Mel and K+, Na+, ATP revealed that inhibitory effect of Mel was competitive with K+, but not with Na+ and ATP. CONCLUSION: Mel polypeptide potently inhibits Na+, K+-ATPase, possibly by binding to the K+ site.

AB - AIM: To investigate the effect of melittin (Mel) of Na+, K+-ATPase activity and it's kinetic mode of action on guinea pig myocardial mitochondria. METHODS: Effect of Mel on heart mitochondrial Na+, K+-ATPase activity was determined with colorimetry method. RESULTS: Mel inhibited Na+, K+-ATPase in a concentration and time dependent manner, IC50 was 2.60 μmol/L. Kinetic studies of interaction between Mel and K+, Na+, ATP revealed that inhibitory effect of Mel was competitive with K+, but not with Na+ and ATP. CONCLUSION: Mel polypeptide potently inhibits Na+, K+-ATPase, possibly by binding to the K+ site.

KW - Bee venoms

KW - Melittin

KW - Mitochondria

KW - Myocardium

KW - Na, K-exchanging ATPase

UR - https://www.scopus.com/pages/publications/0035107948

M3 - 文章

C2 - 11742578

AN - SCOPUS:0035107948

SN - 0253-9756

VL - 22

SP - 279

EP - 282

JO - Acta Pharmacologica Sinica

JF - Acta Pharmacologica Sinica

IS - 3

ER -

Inhibitory effect of melittin on Na⁺, K⁺-ATPase from guinea pig myocardial mitochondria

Abstract

Keywords

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