Inducible protein knockout reveals temporal requirement of CaMKII reactivation for memory consolidation in the brain

Huimin Wang; Eiji Shimizu; Ya Ping Tang; Min Cho; Maureen Kyin; Wenqi Zuo; Daphne A. Robinson; Peter J. Alaimo; Chao Zhang; Hiromi Morimoto; Min Zhuo; Ruiben Feng; Kevan M. Shokat; Joe Z. Tsien

doi:10.1073/pnas.0636870100

Inducible protein knockout reveals temporal requirement of CaMKII reactivation for memory consolidation in the brain

Huimin Wang, Eiji Shimizu, Ya Ping Tang, Min Cho, Maureen Kyin, Wenqi Zuo, Daphne A. Robinson, Peter J. Alaimo, Chao Zhang, Hiromi Morimoto, Min Zhuo, Ruiben Feng, Kevan M. Shokat, Joe Z. Tsien

Research output: Contribution to journal › Article › peer-review

141 Scopus citations

Abstract

By integrating convergent protein engineering and rational inhibitor design, we have developed an in vivo conditional protein knockout and/or manipulation technology. This method is based on the creation of a specific interaction interface between a modified protein domain and sensitized inhibitors. By introducing this system into genetically modified mice, we can readily manipulate the activity of a targeted protein, such as α-Ca²⁺/calmodulin-dependent protein kinase II (αCAMKII), on the time scale of minutes in specific brain subregions of freely behaving mice. With this inducible and region-specific protein knockout technique, we analyzed the temporal stages of memory consolidation process and revealed the first postlearning week as the critical time window during which a precise level of CaMKII reactivation is essential for the consolidation of long-term memories in the brain.

Original language	English
Pages (from-to)	4287-4292
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	100
Issue number	7
DOIs	https://doi.org/10.1073/pnas.0636870100
State	Published - 1 Apr 2003
Externally published	Yes

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Wang, H., Shimizu, E., Tang, Y. P., Cho, M., Kyin, M., Zuo, W., Robinson, D. A., Alaimo, P. J., Zhang, C., Morimoto, H., Zhuo, M., Feng, R., Shokat, K. M., & Tsien, J. Z. (2003). Inducible protein knockout reveals temporal requirement of CaMKII reactivation for memory consolidation in the brain. Proceedings of the National Academy of Sciences of the United States of America, 100(7), 4287-4292. https://doi.org/10.1073/pnas.0636870100

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title = "Inducible protein knockout reveals temporal requirement of CaMKII reactivation for memory consolidation in the brain",

abstract = "By integrating convergent protein engineering and rational inhibitor design, we have developed an in vivo conditional protein knockout and/or manipulation technology. This method is based on the creation of a specific interaction interface between a modified protein domain and sensitized inhibitors. By introducing this system into genetically modified mice, we can readily manipulate the activity of a targeted protein, such as α-Ca2+/calmodulin-dependent protein kinase II (αCAMKII), on the time scale of minutes in specific brain subregions of freely behaving mice. With this inducible and region-specific protein knockout technique, we analyzed the temporal stages of memory consolidation process and revealed the first postlearning week as the critical time window during which a precise level of CaMKII reactivation is essential for the consolidation of long-term memories in the brain.",

author = "Huimin Wang and Eiji Shimizu and Tang, \{Ya Ping\} and Min Cho and Maureen Kyin and Wenqi Zuo and Robinson, \{Daphne A.\} and Alaimo, \{Peter J.\} and Chao Zhang and Hiromi Morimoto and Min Zhuo and Ruiben Feng and Shokat, \{Kevan M.\} and Tsien, \{Joe Z.\}",

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doi = "10.1073/pnas.0636870100",

language = "英语",

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Wang, H, Shimizu, E, Tang, YP, Cho, M, Kyin, M, Zuo, W, Robinson, DA, Alaimo, PJ, Zhang, C, Morimoto, H, Zhuo, M, Feng, R, Shokat, KM & Tsien, JZ 2003, 'Inducible protein knockout reveals temporal requirement of CaMKII reactivation for memory consolidation in the brain', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 7, pp. 4287-4292. https://doi.org/10.1073/pnas.0636870100

TY - JOUR

T1 - Inducible protein knockout reveals temporal requirement of CaMKII reactivation for memory consolidation in the brain

AU - Wang, Huimin

AU - Shimizu, Eiji

AU - Tang, Ya Ping

AU - Cho, Min

AU - Kyin, Maureen

AU - Zuo, Wenqi

AU - Robinson, Daphne A.

AU - Alaimo, Peter J.

AU - Zhang, Chao

AU - Morimoto, Hiromi

AU - Zhuo, Min

AU - Feng, Ruiben

AU - Shokat, Kevan M.

AU - Tsien, Joe Z.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - By integrating convergent protein engineering and rational inhibitor design, we have developed an in vivo conditional protein knockout and/or manipulation technology. This method is based on the creation of a specific interaction interface between a modified protein domain and sensitized inhibitors. By introducing this system into genetically modified mice, we can readily manipulate the activity of a targeted protein, such as α-Ca2+/calmodulin-dependent protein kinase II (αCAMKII), on the time scale of minutes in specific brain subregions of freely behaving mice. With this inducible and region-specific protein knockout technique, we analyzed the temporal stages of memory consolidation process and revealed the first postlearning week as the critical time window during which a precise level of CaMKII reactivation is essential for the consolidation of long-term memories in the brain.

AB - By integrating convergent protein engineering and rational inhibitor design, we have developed an in vivo conditional protein knockout and/or manipulation technology. This method is based on the creation of a specific interaction interface between a modified protein domain and sensitized inhibitors. By introducing this system into genetically modified mice, we can readily manipulate the activity of a targeted protein, such as α-Ca2+/calmodulin-dependent protein kinase II (αCAMKII), on the time scale of minutes in specific brain subregions of freely behaving mice. With this inducible and region-specific protein knockout technique, we analyzed the temporal stages of memory consolidation process and revealed the first postlearning week as the critical time window during which a precise level of CaMKII reactivation is essential for the consolidation of long-term memories in the brain.

UR - https://www.scopus.com/pages/publications/0037388214

U2 - 10.1073/pnas.0636870100

DO - 10.1073/pnas.0636870100

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AN - SCOPUS:0037388214

SN - 0027-8424

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SP - 4287

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 7

ER -

Inducible protein knockout reveals temporal requirement of CaMKII reactivation for memory consolidation in the brain

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