In vivo monitoring of the thiols in rat striatum by liquid chromatography with amperometric detection at a functionalized multi-wall carbon nanotubes modified electrode

Xu Ni Cao; Li Lin; Yue Zhong Xian; Wen Zhang; Yun Feng Xie; Li Tong Jin

doi:10.1002/elan.200390111

In vivo monitoring of the thiols in rat striatum by liquid chromatography with amperometric detection at a functionalized multi-wall carbon nanotubes modified electrode

Xu Ni Cao
, Li Lin
, Yue Zhong Xian
, Wen Zhang
, Yun Feng Xie
, Li Tong Jin^*

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A new chemically modified electrode (CME) was fabricated, which was based on the immobilization of multi-wall carbon nanotubes fuctionalized with carboxylic group (MWNT-COOH). The results indicated that the CME exhibited efficiently electrocatalytic oxidation for L-cysteine and glutathione with relatively high sensitivity, stability and long-life. Coupled with HPLC, the MWNT-COOH CME was utilized for amperometric detection of the thiols. The peak currents of L-cysteine and glutathione were linear to their concentrations ranging from 3.0 × 10^-7 to 1.0 × 10^-3 mol/L with the calculated detection limit (S/N = 3) of 1.2 × 10^-7, 2.2 × 10^-7 mol/L, respectively. The method had been successfully applied to assess the contents of L-cysteine and glutathione in rat striatal microdialysates.

Original language	English
Pages (from-to)	892-897
Number of pages	6
Journal	Electroanalysis
Volume	15
Issue number	10
DOIs	https://doi.org/10.1002/elan.200390111
State	Published - Jul 2003

Keywords

Chemically modified electrode
Functionlized multi-wall carbon nanotubes
HPLC-ECD
Microdialysis
Rat striatum
Thiols

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10.1002/elan.200390111

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@article{18e2631731e84a1d92a90c262e0bdf8d,

title = "In vivo monitoring of the thiols in rat striatum by liquid chromatography with amperometric detection at a functionalized multi-wall carbon nanotubes modified electrode",

abstract = "A new chemically modified electrode (CME) was fabricated, which was based on the immobilization of multi-wall carbon nanotubes fuctionalized with carboxylic group (MWNT-COOH). The results indicated that the CME exhibited efficiently electrocatalytic oxidation for L-cysteine and glutathione with relatively high sensitivity, stability and long-life. Coupled with HPLC, the MWNT-COOH CME was utilized for amperometric detection of the thiols. The peak currents of L-cysteine and glutathione were linear to their concentrations ranging from 3.0 × 10-7 to 1.0 × 10-3 mol/L with the calculated detection limit (S/N = 3) of 1.2 × 10-7, 2.2 × 10-7 mol/L, respectively. The method had been successfully applied to assess the contents of L-cysteine and glutathione in rat striatal microdialysates.",

keywords = "Chemically modified electrode, Functionlized multi-wall carbon nanotubes, HPLC-ECD, Microdialysis, Rat striatum, Thiols",

author = "Cao, \{Xu Ni\} and Li Lin and Xian, \{Yue Zhong\} and Wen Zhang and Xie, \{Yun Feng\} and Jin, \{Li Tong\}",

year = "2003",

month = jul,

doi = "10.1002/elan.200390111",

language = "英语",

volume = "15",

pages = "892--897",

journal = "Electroanalysis",

issn = "1040-0397",

publisher = "Wiley-VCH Verlag",

number = "10",

}

TY - JOUR

T1 - In vivo monitoring of the thiols in rat striatum by liquid chromatography with amperometric detection at a functionalized multi-wall carbon nanotubes modified electrode

AU - Cao, Xu Ni

AU - Lin, Li

AU - Xian, Yue Zhong

AU - Zhang, Wen

AU - Xie, Yun Feng

AU - Jin, Li Tong

PY - 2003/7

Y1 - 2003/7

N2 - A new chemically modified electrode (CME) was fabricated, which was based on the immobilization of multi-wall carbon nanotubes fuctionalized with carboxylic group (MWNT-COOH). The results indicated that the CME exhibited efficiently electrocatalytic oxidation for L-cysteine and glutathione with relatively high sensitivity, stability and long-life. Coupled with HPLC, the MWNT-COOH CME was utilized for amperometric detection of the thiols. The peak currents of L-cysteine and glutathione were linear to their concentrations ranging from 3.0 × 10-7 to 1.0 × 10-3 mol/L with the calculated detection limit (S/N = 3) of 1.2 × 10-7, 2.2 × 10-7 mol/L, respectively. The method had been successfully applied to assess the contents of L-cysteine and glutathione in rat striatal microdialysates.

AB - A new chemically modified electrode (CME) was fabricated, which was based on the immobilization of multi-wall carbon nanotubes fuctionalized with carboxylic group (MWNT-COOH). The results indicated that the CME exhibited efficiently electrocatalytic oxidation for L-cysteine and glutathione with relatively high sensitivity, stability and long-life. Coupled with HPLC, the MWNT-COOH CME was utilized for amperometric detection of the thiols. The peak currents of L-cysteine and glutathione were linear to their concentrations ranging from 3.0 × 10-7 to 1.0 × 10-3 mol/L with the calculated detection limit (S/N = 3) of 1.2 × 10-7, 2.2 × 10-7 mol/L, respectively. The method had been successfully applied to assess the contents of L-cysteine and glutathione in rat striatal microdialysates.

KW - Chemically modified electrode

KW - Functionlized multi-wall carbon nanotubes

KW - HPLC-ECD

KW - Microdialysis

KW - Rat striatum

KW - Thiols

UR - https://www.scopus.com/pages/publications/0042769165

U2 - 10.1002/elan.200390111

DO - 10.1002/elan.200390111

M3 - 文章

AN - SCOPUS:0042769165

SN - 1040-0397

VL - 15

SP - 892

EP - 897

JO - Electroanalysis

JF - Electroanalysis

IS - 10

ER -

In vivo monitoring of the thiols in rat striatum by liquid chromatography with amperometric detection at a functionalized multi-wall carbon nanotubes modified electrode

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Keywords

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