TY - JOUR
T1 - In vivo determination of the monoamine neurotransmitters in rat brain by liquid chromatography with a thioctic acid/iridium oxide-palladium modified electrode
AU - Zhang, Wen
AU - Cao, Xuni
AU - Wan, Fangli
AU - Zhang, Song
AU - Jin, Litong
PY - 2002/11/20
Y1 - 2002/11/20
N2 - The fabrication and application of a electrochemical detector (ED) with the thioctic acid (TA)/iridium oxide (IrO2)-palladium (Pd) chemically modified electrode (CME) for liquid chromatography (LC) were described. The electrochemical behavior of dopamine (DA) and ascorbic acid (AA) at the TA/IrO2-Pd CME were investigated by differential pulse voltammetry (DPV). It was found that the CME could be permselective to DA cations and exile the AA anions, which could be used to determine the monoamine neurotransmitters and avoid the interference of AA in LC. In liquid chromatography with electrochemical detection (LC-ED), DA, norepinephrine (NE), 5-hydroxytryptamine (5-HT), epinephrine (E) and 3,4-dihydroxyphenylactic acid (DOPAC) were determined at the CME. The linear ranges of five analytes were over three orders magnitude and the limits of detection were 0.05pmol for DA, 0.05pmol for NE, 0.05pmol for E, 0.10pmol for 5-HT and 1.00pmol for DOPAC. The application of this method coupled with microdialysis sampling for the in vivo determination of the monoamines in rat brain was also investigated.
AB - The fabrication and application of a electrochemical detector (ED) with the thioctic acid (TA)/iridium oxide (IrO2)-palladium (Pd) chemically modified electrode (CME) for liquid chromatography (LC) were described. The electrochemical behavior of dopamine (DA) and ascorbic acid (AA) at the TA/IrO2-Pd CME were investigated by differential pulse voltammetry (DPV). It was found that the CME could be permselective to DA cations and exile the AA anions, which could be used to determine the monoamine neurotransmitters and avoid the interference of AA in LC. In liquid chromatography with electrochemical detection (LC-ED), DA, norepinephrine (NE), 5-hydroxytryptamine (5-HT), epinephrine (E) and 3,4-dihydroxyphenylactic acid (DOPAC) were determined at the CME. The linear ranges of five analytes were over three orders magnitude and the limits of detection were 0.05pmol for DA, 0.05pmol for NE, 0.05pmol for E, 0.10pmol for 5-HT and 1.00pmol for DOPAC. The application of this method coupled with microdialysis sampling for the in vivo determination of the monoamines in rat brain was also investigated.
KW - LC-ED
KW - Microdialysis sampling
KW - Neurotransmitter
KW - Thioctic acid/iridium oxide-palladium modified electrode
UR - https://www.scopus.com/pages/publications/0037145762
U2 - 10.1016/S0003-2670(02)00764-X
DO - 10.1016/S0003-2670(02)00764-X
M3 - 文章
AN - SCOPUS:0037145762
SN - 0003-2670
VL - 472
SP - 27
EP - 35
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1-2
ER -