TY - JOUR
T1 - In vitro panning of a targeting peptide to hepatocarcinoma from a phage display peptide library
AU - Du, Bing
AU - Qian, Min
AU - Zhou, Zhongliang
AU - Wang, Peng
AU - Wang, Lei
AU - Zhang, Xiaoping
AU - Wu, Miao
AU - Zhang, Ping
AU - Mei, Bing
PY - 2006/4/14
Y1 - 2006/4/14
N2 - Phage display technology has been used as a powerful tool in the discovery of ligands specific to receptor(s) on the surface of a cancer cell and could also impact clinical issues including functional diagnosis and cell-specific drug delivery. After three rounds of in vitro panning and two rounds of reverse absorption, a group of phages capable of addressing BEL-7402 enormously were obtained for further analysis. Through a cell-based ELISA, immunofluorescence, FACS, and in vivo binding study, WP05 (sequence TACHQHVRMVRP) was demonstrated to be the most effective peptide in targeting four kinds of liver cancer cell lines (BEL-7402, BEL-7404, SMMC-7721, and HepG2), but not the normal liver cell line HL-7702. In conclusion, the peptide WP05 which was screened by in vitro phage display technology was proved to be a targeting peptide to several common hepatocellular carcinoma cell lines.
AB - Phage display technology has been used as a powerful tool in the discovery of ligands specific to receptor(s) on the surface of a cancer cell and could also impact clinical issues including functional diagnosis and cell-specific drug delivery. After three rounds of in vitro panning and two rounds of reverse absorption, a group of phages capable of addressing BEL-7402 enormously were obtained for further analysis. Through a cell-based ELISA, immunofluorescence, FACS, and in vivo binding study, WP05 (sequence TACHQHVRMVRP) was demonstrated to be the most effective peptide in targeting four kinds of liver cancer cell lines (BEL-7402, BEL-7404, SMMC-7721, and HepG2), but not the normal liver cell line HL-7702. In conclusion, the peptide WP05 which was screened by in vitro phage display technology was proved to be a targeting peptide to several common hepatocellular carcinoma cell lines.
KW - Hepatocellular carcinoma
KW - In vitro
KW - Phage display library
KW - Targeting peptides
UR - https://www.scopus.com/pages/publications/33644620264
U2 - 10.1016/j.bbrc.2006.02.050
DO - 10.1016/j.bbrc.2006.02.050
M3 - 文章
C2 - 16598852
AN - SCOPUS:33644620264
SN - 0006-291X
VL - 342
SP - 956
EP - 962
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
