TY - JOUR
T1 - In-situ monitoring of signal transduction between skin cells induced by UVB irradiation using scanning electrochemical microscopy
AU - Jing, Ting
AU - Wu, Tao
AU - Lu, Yuqi
AU - Zhang, Fan
AU - Wang, Qingjiang
AU - He, Pingang
N1 - Publisher Copyright:
© 2023
PY - 2023/9/1
Y1 - 2023/9/1
N2 - Skin is the first barrier of the immune system, protecting the body from various damages of the external environment, the signal transduction between skin cells plays an important role in skin healing and injury. Scanning electrochemical microscopy (SECM) combined with a Transwell co-culture device has been proposed to in-situ investigate the signal transduction between skin cells with UVB irradiation, in which, keratinocyte HaCaT cells and fibroblast HFF cells were used as signaling cells and signal-receiving cells, respectively. Extracellular ROS and pH, cell membrane permeability and cell height were in-situ monitored by multi-potential step waveform in SECM (SECM-MPSW), potentiometric mode, shear force mode and the probe approach curve (PAC) combined with COMSOL simulation, respectively. The results revealed that UVB irradiation stimulated HaCaT cells to release excess H+, which diffused to HFF cells and increased the ROS release and membrane permeability with unchanged cell height. Therefore, this work provides an effective way to in-situ investigate intercellular signal transduction.
AB - Skin is the first barrier of the immune system, protecting the body from various damages of the external environment, the signal transduction between skin cells plays an important role in skin healing and injury. Scanning electrochemical microscopy (SECM) combined with a Transwell co-culture device has been proposed to in-situ investigate the signal transduction between skin cells with UVB irradiation, in which, keratinocyte HaCaT cells and fibroblast HFF cells were used as signaling cells and signal-receiving cells, respectively. Extracellular ROS and pH, cell membrane permeability and cell height were in-situ monitored by multi-potential step waveform in SECM (SECM-MPSW), potentiometric mode, shear force mode and the probe approach curve (PAC) combined with COMSOL simulation, respectively. The results revealed that UVB irradiation stimulated HaCaT cells to release excess H+, which diffused to HFF cells and increased the ROS release and membrane permeability with unchanged cell height. Therefore, this work provides an effective way to in-situ investigate intercellular signal transduction.
KW - In-situ investigation
KW - Intercellular signal transduction
KW - Scannig electrochemical microscopy (SECM)
KW - Skin cells
UR - https://www.scopus.com/pages/publications/85161062791
U2 - 10.1016/j.electacta.2023.142693
DO - 10.1016/j.electacta.2023.142693
M3 - 文章
AN - SCOPUS:85161062791
SN - 0013-4686
VL - 461
JO - Electrochimica Acta
JF - Electrochimica Acta
M1 - 142693
ER -