TY - JOUR
T1 - In Situ Monitoring of Hydrogen Peroxide Released from Living Cells Using a ZIF-8-Based Surface-Enhanced Raman Scattering Sensor
AU - Jiang, Lei
AU - He, Cai Hong
AU - Chen, Hua Ying
AU - Xi, Cheng Ye
AU - Fodjo, Essy Kouadio
AU - Zhou, Ze Rui
AU - Qian, Ruo Can
AU - Li, Da Wei
AU - Hafez, Mahmoud Elsayed
N1 - Publisher Copyright:
© 2021 American Chemical Society
PY - 2021/9/21
Y1 - 2021/9/21
N2 - Hydrogen peroxide (H2O2) widely involves in intracellular and intercellular redox signaling pathways, playing a vital role in regulating various physiological events. Nevertheless, current analytical methods for the H2O2assay are often hindered by relatively long response time, low sensitivity, or self-interference. Herein, a zeolitic imidazolate framework-8 (ZIF-8)-based surface-enhanced Raman scattering (SERS) sensor has been developed to detect H2O2released from living cells by depositing ZIF-8 over SERS active gold nanoparticles (AuNPs) grafted with H2O2-responsive probe molecules, 2-mercaptohydroquinone. Combining the superior fingerprint identification of SERS and the highly efficient enrichment and selective response of H2O2by ZIF, the ZIF-8-based SERS sensor exhibits a high anti-interference ability for H2O2detection, with a limit of detection as low as 0.357 nM. Satisfyingly, owing to the enhanced catalytic activity derived from the successful integration of AuNPs and ZIF, the response time as short as 1 min can be obtained, demonstrating the effectiveness of the SERS sensor for rapid H2O2detection. Furthermore, the developed SERS sensor enables real-time detection of H2O2secreted from living cells under phorbol myristate acetate stimulation, as cells can be cultured on-chip. This study will pave the way toward the development of a metal-organic framework-based SERS platform for application in the fields of biosensing and early disease diagnosis associated with H2O2secretion, thus exhibiting promising potential for future therapies.
AB - Hydrogen peroxide (H2O2) widely involves in intracellular and intercellular redox signaling pathways, playing a vital role in regulating various physiological events. Nevertheless, current analytical methods for the H2O2assay are often hindered by relatively long response time, low sensitivity, or self-interference. Herein, a zeolitic imidazolate framework-8 (ZIF-8)-based surface-enhanced Raman scattering (SERS) sensor has been developed to detect H2O2released from living cells by depositing ZIF-8 over SERS active gold nanoparticles (AuNPs) grafted with H2O2-responsive probe molecules, 2-mercaptohydroquinone. Combining the superior fingerprint identification of SERS and the highly efficient enrichment and selective response of H2O2by ZIF, the ZIF-8-based SERS sensor exhibits a high anti-interference ability for H2O2detection, with a limit of detection as low as 0.357 nM. Satisfyingly, owing to the enhanced catalytic activity derived from the successful integration of AuNPs and ZIF, the response time as short as 1 min can be obtained, demonstrating the effectiveness of the SERS sensor for rapid H2O2detection. Furthermore, the developed SERS sensor enables real-time detection of H2O2secreted from living cells under phorbol myristate acetate stimulation, as cells can be cultured on-chip. This study will pave the way toward the development of a metal-organic framework-based SERS platform for application in the fields of biosensing and early disease diagnosis associated with H2O2secretion, thus exhibiting promising potential for future therapies.
UR - https://www.scopus.com/pages/publications/85115795426
U2 - 10.1021/acs.analchem.1c02233
DO - 10.1021/acs.analchem.1c02233
M3 - 文章
AN - SCOPUS:85115795426
SN - 0003-2700
VL - 93
SP - 12609
EP - 12616
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 37
ER -