TY - JOUR
T1 - Improvement of hydrogen yield of lba-transgenic Chlamydomonas reinhardtii caused by increasing respiration and impairing photosynthesis
AU - Xu, Lili
AU - Wang, Quanxi
AU - Wu, Shuangxiu
AU - Li, Dezhi
PY - 2014/8/22
Y1 - 2014/8/22
N2 - The transgenic alga lba of Chlamydomonas reinhardtii yielded H2 with 50%-180% higher than the control strain. Further experiments showed that photosynthetic rates and photosynthetic reaction center II's photochemical capacities of the transgenic algae obviously decreased 33.4%-85.9% and 30.0%-51.7%, respectively, compared with those of the control. On the contrary, respiration rates of the transgenic algae significantly increased, with 40.0%-200.0% higher than those of the control. Furthermore, starch contents of the transgenic algae were also improved significantly by 79.1%-592.8% compared with the control. Therefore, the reason of H2 yield improvement of the transgenic alga lba is not only due to its decrease of photosynthetic capacity and increase of the respiration rate, but also due to the metabolic changes related to starch metabolism, photosynthesis and respiration which is possibly caused by hetero-expression of lba gene in chloroplasts of C. reinhardtii, indicating the potential of utilization of lba gene to improve hydrogen yield of micro-green algae.
AB - The transgenic alga lba of Chlamydomonas reinhardtii yielded H2 with 50%-180% higher than the control strain. Further experiments showed that photosynthetic rates and photosynthetic reaction center II's photochemical capacities of the transgenic algae obviously decreased 33.4%-85.9% and 30.0%-51.7%, respectively, compared with those of the control. On the contrary, respiration rates of the transgenic algae significantly increased, with 40.0%-200.0% higher than those of the control. Furthermore, starch contents of the transgenic algae were also improved significantly by 79.1%-592.8% compared with the control. Therefore, the reason of H2 yield improvement of the transgenic alga lba is not only due to its decrease of photosynthetic capacity and increase of the respiration rate, but also due to the metabolic changes related to starch metabolism, photosynthesis and respiration which is possibly caused by hetero-expression of lba gene in chloroplasts of C. reinhardtii, indicating the potential of utilization of lba gene to improve hydrogen yield of micro-green algae.
KW - Bio-hydrogen production
KW - Chlamydomonas reinhardtii
KW - Photosynthetic activity
KW - Respiration rate
KW - Starch
KW - lba gene
UR - https://www.scopus.com/pages/publications/84905915106
U2 - 10.1016/j.ijhydene.2014.04.178
DO - 10.1016/j.ijhydene.2014.04.178
M3 - 文章
AN - SCOPUS:84905915106
SN - 0360-3199
VL - 39
SP - 13347
EP - 13352
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 25
ER -
