Improved resolution in fluorescence microscopy with the FRET pairs by time gating

Shangguo Hou; Jianfang Chen; Suhui Deng; Fei Wang; Qing Huang; Ya Cheng; Chunhai Fan

doi:10.1364/OE.23.013121

Improved resolution in fluorescence microscopy with the FRET pairs by time gating

Shangguo Hou, Jianfang Chen, Suhui Deng, Fei Wang, Qing Huang, Ya Cheng, Chunhai Fan

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We investigate the temporal information of nonlinear donor fluorescence in the FRET frustration microscopy. The arrival time of the donor fluorescence varies with the distribution of the excitation laser. The differences of the arrival times between different positions in the excitation spot can be further enhanced in the case of adding a depletion beam upon the FRET probes. The spatial information is encoded in the temporal dynamics of the fluorescent photons from donor molecules and time-gating in detection can be used to increase the spatial resolution.

Original language	English
Pages (from-to)	13121-13129
Number of pages	9
Journal	Optics Express
Volume	23
Issue number	10
DOIs	https://doi.org/10.1364/OE.23.013121
State	Published - 2015
Externally published	Yes

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10.1364/OE.23.013121

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title = "Improved resolution in fluorescence microscopy with the FRET pairs by time gating",

abstract = "We investigate the temporal information of nonlinear donor fluorescence in the FRET frustration microscopy. The arrival time of the donor fluorescence varies with the distribution of the excitation laser. The differences of the arrival times between different positions in the excitation spot can be further enhanced in the case of adding a depletion beam upon the FRET probes. The spatial information is encoded in the temporal dynamics of the fluorescent photons from donor molecules and time-gating in detection can be used to increase the spatial resolution.",

author = "Shangguo Hou and Jianfang Chen and Suhui Deng and Fei Wang and Qing Huang and Ya Cheng and Chunhai Fan",

note = "Publisher Copyright: {\textcopyright}2015 Optical Society of America.",

year = "2015",

doi = "10.1364/OE.23.013121",

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T1 - Improved resolution in fluorescence microscopy with the FRET pairs by time gating

AU - Hou, Shangguo

AU - Chen, Jianfang

AU - Deng, Suhui

AU - Wang, Fei

AU - Huang, Qing

AU - Cheng, Ya

AU - Fan, Chunhai

PY - 2015

Y1 - 2015

N2 - We investigate the temporal information of nonlinear donor fluorescence in the FRET frustration microscopy. The arrival time of the donor fluorescence varies with the distribution of the excitation laser. The differences of the arrival times between different positions in the excitation spot can be further enhanced in the case of adding a depletion beam upon the FRET probes. The spatial information is encoded in the temporal dynamics of the fluorescent photons from donor molecules and time-gating in detection can be used to increase the spatial resolution.

AB - We investigate the temporal information of nonlinear donor fluorescence in the FRET frustration microscopy. The arrival time of the donor fluorescence varies with the distribution of the excitation laser. The differences of the arrival times between different positions in the excitation spot can be further enhanced in the case of adding a depletion beam upon the FRET probes. The spatial information is encoded in the temporal dynamics of the fluorescent photons from donor molecules and time-gating in detection can be used to increase the spatial resolution.

UR - https://www.scopus.com/pages/publications/84946593226

U2 - 10.1364/OE.23.013121

DO - 10.1364/OE.23.013121

M3 - 文章

AN - SCOPUS:84946593226

SN - 1094-4087

VL - 23

SP - 13121

EP - 13129

JO - Optics Express

JF - Optics Express

IS - 10

ER -

Improved resolution in fluorescence microscopy with the FRET pairs by time gating

Abstract

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