Abstract
tIn this work, we report the development of a highly sensitive and stable choline/acetylcholine sensorbased on the synergetic effect of multi-walled carbon nanotubes and ZnO nanoparticles. This uniquemulti-layer structure provided a favorable microenvironment to maintain the bioactivity of choline oxi-dase and acetylcholinesterase, which led to a rapid amperometric response to choline and acetylcholine.This nanomaterials-based choline/acetylcholine sensor was highly sensitive and showed good stabilityover a relatively long-term storage (90 days). The sensor prepared by loading 1.0-unit choline oxidaseand acetylcholinesterase showed a linear response range of 1.0 μM to 0.8 mM and 1.0 μM to 1.0 mM, asensitivity of 178 μA mM-1cm-2and 180 μA mM-1cm-2, and a detection limit of 0.3 μM, respectively.The sensor prepared was used to detect choline in human blood plasma samples, and the obtained resultswere consistent with those of the HPLC-MS/MS assay.
| Original language | English |
|---|---|
| Pages (from-to) | 904-910 |
| Number of pages | 7 |
| Journal | Sensors and Actuators B: Chemical |
| Volume | 193 |
| DOIs | |
| State | Published - 31 Mar 2014 |
Keywords
- Acetylcholine biosensor
- Choline biosensor
- Multi-wall carbon nanotubes
- Synergic actiona
- ZnO nanoparticles