Improved enzyme immobilization for enhanced bioelectrocatalytic activity of choline sensor

Yiting Wang; Lei Yu; Ziqiang Zhu; Jing Wang; Yanjie Bao; Weijie Du; Junjun Li; Hui Peng; Jianzhong Zhu

doi:10.1166/nnl.2013.1590

Improved enzyme immobilization for enhanced bioelectrocatalytic activity of choline sensor

Yiting Wang, Lei Yu, Ziqiang Zhu, Jing Wang, Yanjie Bao, Weijie Du, Junjun Li, Hui Peng, Jianzhong Zhu

School of Physics and Electronic Science

East China Normal University

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In this work, we report the development of a highly sensitive and stable choline sensor based on the synergetic effect of multi-walled carbon nanotubes and ZnO nanoparticles. This unique multi-layer structure provided a favorable microenvironment to maintain the bioactivity of choline oxidase, which led to a rapid amperometric response to choline. This nanomaterials-based choline sensor was highly sensitive and showed good stability over a relatively long-term storage (90 days). The sensor prepared by loading 1.0-unit choline oxidase showed a linear response range of 1.0 μM to 0.8 mM, a sensitivity of 178 μA mM^-1 cm^-2 and a detection limit of 0.3 μM. The sensor prepared was used to detect choline in human blood plasma samples, and the obtained results were consistent with those of the HPLC-MS/MS assay.

Original language	English
Pages (from-to)	660-665
Number of pages	6
Journal	Nanoscience and Nanotechnology Letters
Volume	5
Issue number	6
DOIs	https://doi.org/10.1166/nnl.2013.1590
State	Published - Jun 2013

Keywords

Choline Biosensor
Choline Oxidase
Multi-Wall Carbon Nanotubes
Synergic Action
ZnO Nanoparticles

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10.1166/nnl.2013.1590

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title = "Improved enzyme immobilization for enhanced bioelectrocatalytic activity of choline sensor",

abstract = "In this work, we report the development of a highly sensitive and stable choline sensor based on the synergetic effect of multi-walled carbon nanotubes and ZnO nanoparticles. This unique multi-layer structure provided a favorable microenvironment to maintain the bioactivity of choline oxidase, which led to a rapid amperometric response to choline. This nanomaterials-based choline sensor was highly sensitive and showed good stability over a relatively long-term storage (90 days). The sensor prepared by loading 1.0-unit choline oxidase showed a linear response range of 1.0 μM to 0.8 mM, a sensitivity of 178 μA mM-1 cm-2 and a detection limit of 0.3 μM. The sensor prepared was used to detect choline in human blood plasma samples, and the obtained results were consistent with those of the HPLC-MS/MS assay.",

keywords = "Choline Biosensor, Choline Oxidase, Multi-Wall Carbon Nanotubes, Synergic Action, ZnO Nanoparticles",

author = "Yiting Wang and Lei Yu and Ziqiang Zhu and Jing Wang and Yanjie Bao and Weijie Du and Junjun Li and Hui Peng and Jianzhong Zhu",

year = "2013",

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AU - Wang, Yiting

AU - Yu, Lei

AU - Zhu, Ziqiang

AU - Wang, Jing

AU - Bao, Yanjie

AU - Du, Weijie

AU - Li, Junjun

AU - Peng, Hui

AU - Zhu, Jianzhong

PY - 2013/6

Y1 - 2013/6

N2 - In this work, we report the development of a highly sensitive and stable choline sensor based on the synergetic effect of multi-walled carbon nanotubes and ZnO nanoparticles. This unique multi-layer structure provided a favorable microenvironment to maintain the bioactivity of choline oxidase, which led to a rapid amperometric response to choline. This nanomaterials-based choline sensor was highly sensitive and showed good stability over a relatively long-term storage (90 days). The sensor prepared by loading 1.0-unit choline oxidase showed a linear response range of 1.0 μM to 0.8 mM, a sensitivity of 178 μA mM-1 cm-2 and a detection limit of 0.3 μM. The sensor prepared was used to detect choline in human blood plasma samples, and the obtained results were consistent with those of the HPLC-MS/MS assay.

AB - In this work, we report the development of a highly sensitive and stable choline sensor based on the synergetic effect of multi-walled carbon nanotubes and ZnO nanoparticles. This unique multi-layer structure provided a favorable microenvironment to maintain the bioactivity of choline oxidase, which led to a rapid amperometric response to choline. This nanomaterials-based choline sensor was highly sensitive and showed good stability over a relatively long-term storage (90 days). The sensor prepared by loading 1.0-unit choline oxidase showed a linear response range of 1.0 μM to 0.8 mM, a sensitivity of 178 μA mM-1 cm-2 and a detection limit of 0.3 μM. The sensor prepared was used to detect choline in human blood plasma samples, and the obtained results were consistent with those of the HPLC-MS/MS assay.

KW - Choline Biosensor

KW - Choline Oxidase

KW - Multi-Wall Carbon Nanotubes

KW - Synergic Action

KW - ZnO Nanoparticles

UR - https://www.scopus.com/pages/publications/84880845966

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DO - 10.1166/nnl.2013.1590

M3 - 文章

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SN - 1941-4900

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SP - 660

EP - 665

JO - Nanoscience and Nanotechnology Letters

JF - Nanoscience and Nanotechnology Letters

IS - 6

ER -

Improved enzyme immobilization for enhanced bioelectrocatalytic activity of choline sensor

Abstract

Keywords

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