Impact of cellulolytic nitrogen-fixing composite inoculants on humification pathways and nitrogen cycling in kitchen waste composting

Low humification and nitrogen loss pose substantial challenges to the resource utilization in kitchen waste composting. This study investigated the effects of brown-rot fungi (BRF), cellulolytic nitrogen fixing bacteria (CNFB), and their composite microbial inoculants (CMI) during composting. Results indicated that microbial inoculants extended the thermophilic phase and enhanced cellulose degradation. Compared with the control, the degree of polymerization (HA/FA) in BRF, CNFB, and CMI was 2.28, 1.85, and 2.68 times higher, respectively, while increasing total nitrogen by 11.15%, 15.50%, and 19.73%. BRF and CMI primarily enhanced the Maillard humification pathway, while CNFB promoted the polyphenol humification pathway. Additionally, BRF enhanced nitrification and reduced denitrification, whereas CNFB and CMI improved nitrification, nitrogen fixation, and ammonification while reducing denitrification. Overall, BRF primarily promoted humification, while CNFB excelled in nitrogen retention. The CMI achieved optimal humification and nitrogen retention, indicating a potential sustainable solution for kitchen waste composting.