Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV

Ningyi Jin; Hongyong Zhang; Gefeng Yin; Min Zheng; Tong Liu; Wenzheng Jiang; Zijian Li

doi:10.1360/03wc0546

Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV

Ningyi Jin^*
, Hongyong Zhang
, Gefeng Yin
, Min Zheng
, Tong Liu
, Wenzheng Jiang
, Zijian Li

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV.

Original language	English
Pages (from-to)	823-827
Number of pages	5
Journal	Chinese Science Bulletin
Volume	49
Issue number	8
DOIs	https://doi.org/10.1360/03wc0546
State	Published - 2004
Externally published	Yes

Keywords

FMDV
Immunogenicity
P1-2A
Recombinant fowl-pox virus (rFPV)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1360/03wc0546

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title = "Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV",

abstract = "Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV.",

keywords = "FMDV, Immunogenicity, P1-2A, Recombinant fowl-pox virus (rFPV)",

author = "Ningyi Jin and Hongyong Zhang and Gefeng Yin and Min Zheng and Tong Liu and Wenzheng Jiang and Zijian Li",

year = "2004",

doi = "10.1360/03wc0546",

language = "英语",

volume = "49",

pages = "823--827",

journal = "Chinese Science Bulletin",

issn = "1001-6538",

publisher = "Elsevier B.V.",

number = "8",

}

TY - JOUR

T1 - Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV

AU - Jin, Ningyi

AU - Zhang, Hongyong

AU - Yin, Gefeng

AU - Zheng, Min

AU - Liu, Tong

AU - Jiang, Wenzheng

AU - Li, Zijian

PY - 2004

Y1 - 2004

N2 - Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV.

AB - Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV.

KW - FMDV

KW - Immunogenicity

KW - P1-2A

KW - Recombinant fowl-pox virus (rFPV)

UR - https://www.scopus.com/pages/publications/33646140093

U2 - 10.1360/03wc0546

DO - 10.1360/03wc0546

M3 - 文章

AN - SCOPUS:33646140093

SN - 1001-6538

VL - 49

SP - 823

EP - 827

JO - Chinese Science Bulletin

JF - Chinese Science Bulletin

IS - 8

ER -

Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV

Abstract

Keywords

UN SDGs

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